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菊芋塊莖提取物對高血脂小鼠干預研究

時間:2019-06-30 07:58來源:畢業論文
利用高脂飼料長期飼喂BALB/c小鼠,建立高脂血癥小鼠模型,對模型小鼠分別采用9.0‰生理鹽水(模型對照組)、低、中、高3種劑量的菊粉和藥物辛伐他汀(藥物對照組)處理

摘要:本試驗利用高脂飼料長期飼喂BALB/c小鼠,建立高脂血癥小鼠模型,對模型小鼠分別采用9.0‰生理鹽水(模型對照組)、低、中、高3種劑量的菊粉和藥物辛伐他汀(藥物對照組)處理,同時用、普通飼料喂養的小鼠作為空白對照,通過測定小鼠的體重、肝體比、脂體比、飼料食用量和血脂指標(總膽固醇、甘油三酯、高密度脂蛋白膽固醇、低密度脂蛋白膽固醇),并觀察肝臟油紅O染色切片分析腸道微生物群落結構以及多樣性,研究了菊粉對高血脂小鼠的緩解作用。結果如下:菊粉組體重總體較為穩定;隨菊粉劑量升高,各劑量菊粉組小鼠肝體比下降;總膽固醇、HDL-C、LDL-C含量都下降,甘油三酯下降效果不明顯;切片結果顯示菊粉組小鼠肝臟的脂肪滴數量少于模型對照組;微生物組成結構有所改變。研究結果表明菊粉對緩解高脂血癥有積極影響。36750
畢業論文關鍵詞:菊粉;高脂血癥;膽固醇;腸道微生物
Study on the effect of Jerusalem artichoke tuber extract on mice with hyperlipidemia
Abstract: In order to investigate the mitigation effect of inulin on mice with hyperlipidemia, the hyperlipidemia mice model was established using high-fat feed. Fifty model mice were randomly and equally pided into model group: low inulin dose group, middle inulin dose group, high inulin dose group and simvastatin group to receive normal saline, low dose of inulin, middle dose of inulin, high dose of inulin and simvastatin, respectively, by gavage. Meanwhile, ten mice were selected to be as blank control group, which were fed with fundamental feed and received normal saline. The changes in body weight, hepatopancreas index, triglyceride (TG), total cholesterol (TC), high and low density lipoprotein cholesterol (HDL-C and LDL-C) were evaluated and the liver oil red O staining were compared and community structure and persity of intestinal microorganisms between the four groups. The results were as follows: the weight of the inulin groups were relatively stable; with the increase of inulin powder, the ratio of liver and body of mice in each dose group decreased; total cholesterol, HDL-C, and LDL-C levels decreased; staining showed the number of fat droplets in mice liver was less than model group; the composition and structure of microorganisms have changed. The results show that inulin can alleviate hyperlipidemia mice obesity, reduce ratio of liver weight and body weight, reduce liver fat accumulation. And the level of cholesterol in serum of hyperlipidemic mice decreased.It also reduce LDL cholesterol in serum,,improve the intestinal microbial population structure and persity. This study showed that Inulin have mitigation effect for mice with hyperlipidemia.

源¥自%六:維;論-文'網=www.aftnzs.live


Key words:  Inulin; Hyperlipidemia; cholesterol; intestinal microflora
目  錄
摘要    1
關鍵詞    1
Abstract    1
Key words     2
引言    2
1材料與方法    3
1.1研究材料    3
1.1.1試驗動物、飼料與試劑    3
1.1.2主要儀器、設備    3
1.2研究方法    4
1.2.1高脂小鼠模型的建立    4
1.2.2重分組及處理    4
1.2.3 各項指標的測定    4
1.2.4肝臟油紅O染色切片    5
1.2.5菊粉對高脂血癥小鼠腸道微生物群落結構以及多樣性的影響    5
1.2.5.1小鼠腸道內容物DNA的提取與純化    5
1.2.5.2 16S rDNA測序實驗流程    5
1.2.5.3分析流程    5
1.3數據統計分析軟件    5
2結果與分析    6
2.1小鼠體重變化    6
2.2肝體比與脂體比    6 菊芋塊莖提取物對高血脂小鼠干預研究:http://www.aftnzs.live/yixue/20190630/35321.html
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