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紫蘇揮發油抗腫瘤活性研究

時間:2019-06-01 09:23來源:畢業論文
植物揮發油是從植物的根、莖、葉或果實中提取出來芳香物質,它用途廣泛,含有多種生物活性物質,許多植物揮發油可以用于生物制藥。有研究表明,一些植物揮發油具有抑制腫瘤細

摘要:植物揮發油是從植物的根、莖、葉或果實中提取出來芳香物質,它用途廣泛,含有多種生物活性物質,許多植物揮發油可以用于生物制藥。有研究表明,一些植物揮發油具有抑制腫瘤細胞生長,誘導細胞凋亡的作用。紫蘇在我國種植廣泛,歷史悠久,在食品和醫藥領域有著重要的價值。本次實驗以來自我國十個不同產地的紫蘇油為研究對象,探究其是否具有抗腫瘤活性及其可能的機制。35826
實驗分為MTT實驗、臺盼藍染色、Western Blot幾個部分。MTT實驗、臺盼藍染色用于檢測紫蘇油對A549細胞活性的影響。實驗結果表明,各個產地的紫蘇油處理細胞后,細胞的死亡率顯著增加。不同產地紫蘇油的處理效果有所差別,其中貴州、甘肅紫蘇油的處理效果最明顯。通過計算各產地紫蘇油的IC50,發現貴州紫蘇油的IC50值最小,僅為0.039 μl/ml。為探究紫蘇油的抗腫瘤機理,進一步用Western Blot方法檢測紫蘇油對凋亡相關蛋白caspase-8、caspase-3、Bcl-2、Bcl-XL、PARP、HSP70、P53等的影響。研究發現HSP70上調,caspase-8、caspase-3酶原被切割,Bcl-2、Bcl-XL下調,P53含量增加,PARP被切割后失活。以上實驗結果表明,紫蘇油具有潛在的抗腫瘤活性,且可以促進腫瘤細胞A549的凋亡。在紫蘇油的作用下,caspase蛋白酶逐漸被切割活化,線粒體外膜通透性增加,啟動外源與內源性的caspase依賴性凋亡途徑,使A549細胞凋亡。
畢業論文關鍵詞:紫蘇油;A549細胞;細胞凋亡;抗腫瘤
Studies on antitumor activity of Perilla frutescens essential oil
Abstract: Plant essential oils are aromatic substances extracted from plant roots, stems, leaves and fruits. These essential oils used widely and have a variety of biological activity, especially used for drugs production. Recently, it has been confirmed that some plant essential oils can inhibit tumor cell growth and induce cell apoptosis. Perilla frutescens were widely planted in our country with a long history. It has high application value in the field of food and medicine. This experiment use P. frutescens essential oils from ten different origins as the experimental subject, to explore whether they have antitumor activity and possible mechanism. 源¥自%六:維;論-文'網=www.aftnzs.live
Experiment is pided into MTT assay, trypan blue dye exclusion assay and western blot. MTT assay and trypan blue dye exclusion assay was used to detect the activity of A549 cells incubated with P. frutescens essential oils. The results show that the mortality rate of cell treated with P. frutescens essential oils increased significantly. Treatment effect of essential oils differs dramatically. Effects of Guizhou and Gansu P. frutescens essential oils are obvious. By calculating IC50 of each essential oil show that IC50 value of Guizhou P. frutescens essential oil is minimum, only 0.039μl/ml. To explore the anti-tumor mechanism of essential oils, we perform western blot to detect the change of protein concentration, such as caspase-8, caspase-3, Bcl-2, Bcl-XL, PARP, HSP70 and P53. HSP70 is increased, caspase-8, caspase-3 zymogens was cut, the concentrations of Bcl-2, Bcl – XL are decreased, P53 is increased, and PARP was cleaved and deactivated. These results showed that essential oils have potential antitumor activity by promoting the apoptosis of tumor cells. Apoptosis of A549 cells was induced by P. frutescens essential oils through the activation of the exogenous and endogenous caspase-depended of apoptotic pathway, which was induced by the activation of the cleaved caspase protease and the increase of the mitochondrial membrane permeability.
Key words: Perilla frutescens essential oils; A549 cell; apoptosis; antitumor
目錄
引言    1
1 材料與方法    2
1.1供試材料    2
1.2細胞    2
1.3試劑    2
1.4細胞培養    2 紫蘇揮發油抗腫瘤活性研究:http://www.aftnzs.live/yixue/20190601/33962.html
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