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糧食及飼料中銅鐵含量的分析研究

時間:2019-06-21 19:37來源:畢業論文
采用分光光度法研究了二乙基二硫代氨基甲酸鈉與銅在堿性環境中的顯色反應和鄰菲羅啉與鐵在乙酸-乙酸鈉緩沖溶液中的顯色反應,確定了最佳試驗條件,提出一個靈敏度高,簡便、快

摘要: 本文采用分光光度法研究了二乙基二硫代氨基甲酸鈉與銅在堿性環境中的顯色反應和鄰菲羅啉與鐵在乙酸-乙酸鈉緩沖溶液中的顯色反應,確定了最佳試驗條件,提出一個靈敏度高,簡便、快捷測定微量銅、鐵的分光光度法。結果表明:在測銅的方法中,我們選擇在pH約為9.0的氯化銨-氫氧化銨緩沖溶液中進行,此時最大吸收波長位于440nm處,最佳顯色時間為5min。回歸方程為:Y=0.06404X,線性范圍為0.2-5ug。而在測鐵的方法中,我們選擇在pH約為5.0的乙酸-乙酸鈉緩沖溶液中進行,此時最大吸收波長位于510nm處,最佳顯色時間為10min。回歸方程為:Y=0.12182X,線性范圍為0.4-1.6ug/ml。此法用于糧食及飼料中微量銅、鐵的測定,獲得了滿意的效果。36400
畢業論文關鍵詞:銅;鐵;分光光度法;二乙基二硫代氨基甲酸鈉;鄰菲羅啉;飼料
The analysis and research on the content of copper and iron in the food and feeds
Abstract: This article makes use of the spectrophotometric method to research the chromogenic reaction on the sodium diethyl dithiocarbamate and copper in the alkaline condition and we also research the chromogenic reaction on the phenanthroline and iron in the buffered solution of acetic acid-sodium acetate. Thus the best experiment conditions were concluded, which will be a high sensitivity, simple and fast spectrophotometric method of copper and iron  testing. Results show that determining the content of copper is fit for the condition of the buffered solution of ammonium chloride-ammonium hydroxide which the pH value is about 9.0, the maximum absorption wavelength is located at 440nm and the best reaction time is 5 minutes.The regression equation is: Y=0.06404X,the linearity range is 0.2-5ug. And determining the content of iron is fit for the condition of the buffered solution of acetic acid-sodium acetate which the pH value is about 5.0, the maximum absorption wavelength is located at 510nm and the best reaction time is 10 minutes.The regression equation is: Y=0.12182X, the linearity range is 0.4-2.0ug/ml. This method can be used for the testing of the content of copper and iron in the food and feeds, and we come off the satisfactory results. 源`自*六:維.論^文'網www.aftnzs.live
Keywords:copper; iron; spectrophotometric method; sodium diethyl dithiocarbamate; phenanthroline; feeds
 目錄
1 前言    1
1.1研究糧食及飼料中銅、鐵離子檢測的重要意義    1
1.2糧食及飼料中銅、鐵含量的檢測方法的介紹    2
1.2.1 分光光度法    2
1.2.2 石墨爐原子吸收光譜法    3
1.2.3 電感耦合等離子體原子發射光譜法    3
1.2.4 火焰原子吸收光譜法    3   
1.3分光光度法的原理    4
1.4分光光度法的分類    5
1.4.1 常規分光光度法    5
1.4.2 催化動力學分光光度法    5
2 實驗內容    6
2.1 試劑與材料    6
2.2 實驗儀器    6
2.3 實驗方法    7
2.3.1 溶液的配制    7
2.3.2 標準曲線的繪制    8
2.3.3 未知樣品的測定    10
3 結果與討論    13
3.1 金屬離子檢測工作條件的確定    11
3.1.1 最大吸收波長的確定    13
3.1.2 顯色劑用量的選擇    16
3.1.3 適宜酸堿度的測定    18
3.1.4 顯色時間的確定    20
3.2 糧食及飼料中銅、鐵離子的檢測方法    22
3.2.1 工作曲線的繪制    22
3.2.2 樣品測定結果    25
4 結論    27 糧食及飼料中銅鐵含量的分析研究:http://www.aftnzs.live/shiping/20190621/34985.html
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