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小米糠中阿拉伯木聚糖的分離提取研究

時間:2019-04-16 20:11來源:畢業論文
在小米糠中,AX主要分為水溶性AX和水不溶性AX兩部分。本論文中,主要研究小米糠中水溶性AX的水提取法和水不溶性AX的酶水解和超聲輔酶水解的提取方法。小米糠中水溶性AX的水提取法

摘要:阿拉伯木聚糖(AX)是谷子細胞壁的主要結構多糖,具有高分子量、高粘度、氧化膠凝等物化特性。在小米糠中,AX主要分為水溶性AX和水不溶性AX兩部分。本論文中,主要研究小米糠中水溶性AX的水提取法和水不溶性AX的酶水解和超聲輔酶水解的提取方法。小米糠中水溶性AX的水提取法中,脫脂小米糠的細度篩選以及物料比(µg/mL)是影響水溶性AX提取率的重要因素。研究表明,脫脂小米糠的細度大于100目時,水溶性AX的提取產率較高;脫脂小米糠的料液比在1:12時水溶性AX的提取產率達到最大。小米糠中水不溶性AX的酶水解和超聲輔酶水解的提取方法中,纖維素酶的作用溫度、pH和時間以及木聚糖酶的添加量是影響水不溶性AX提取率的重要因素。實驗研究表明,溫度為45℃、pH為4.2、酶解時間為3h、木聚糖酶的添加量為180U/g的條件下,水不溶性AX的提取產率最高。在超聲輔酶水解的方法中,超聲與酶水解作用的先后順序對AX的提取產率的影響不顯著。在AX的提取液中含有大量的雜蛋白,運用sevege、硅膠土處理、TCA和TCA-正丁醇結合法四種方法去除AX提取液中的雜蛋白,證明TCA-正丁醇法結合法去除雜蛋白的效果最好。34604
畢業論文關鍵詞:小米糠;阿拉伯木聚糖;纖維素酶;木聚糖酶;超聲輔助法;提取
Study on Isolation of Arabinoxylan from Millet bran
Abstract:Arabian xylan (AX) is the main structural polysaccharide of the millet cell wall, with high molecular weight, high viscosity, oxidative gelation and other physical and chemical properties. In the millet bran, AX is pided into water-soluble AX and water-insoluble AX two parts. In this paper, the water extraction of water-soluble AX and the hydrolysis of water-insoluble AX and the extraction of ultrasonic assisted enzyme were studied. In the water extraction method of water-soluble AX in millet bran, the fineness of defatted millet bran and the ratio of material to liquid of defatted millet bran in water extraction are important factors affecting the extraction rate of water-soluble AX. The results showed that the yield of water-soluble AX was higher when the fineness of defatted millet bran was higher than 100 mesh, and the yield of water-soluble AX was the highest at 1:12. The temperature, pH and time of the cellulase and the amount of xylanase are the important factors influencing the extraction rate of water insoluble AX. The results showed when the temperature was 45 ℃, the pH was 4.2, the time of enzymolysis was 3h, the dosage of xylanase was 180U / g, and the yield of water insoluble AX was the highest. In the method of ultrasonic assisted enzymatic hydrolysis, the order of ultrasound and enzymatic hydrolysis effect on the extraction yield of AX was not significant. AX extract contains a large amount of hybrid protein, then four methods of sevege, silica gel treatment, TCA and TCA-n-butanol were used to remove the protein from AX extract, results showed that TCA-n-butanol method was the best method to remove protein. 源¥自%六:維;論-文'網=www.aftnzs.live
Key words:millet bran; arabinoxylan; cellulase; xylanase; ultrasonic-assisted method;isolation
目錄
摘要    1
關鍵詞    2
Abstract    2
Key words    2
材料與方法    2
1.1材料    2
1.1.1試驗材料    2
1.1.2  主要試劑    2
1.1.3主要儀器設備    3
1.2  小米糠的脫脂處理    3
1.3小米糠中水溶性阿拉伯木聚糖的提取    3
1.3.1脫脂小米糠細度的篩選    3
1.3.2小米糠料液比的篩選    3
1.4小米糠中水不溶性阿拉伯木聚糖的分離提取研究[4]    4
1.4.1纖維素酶酶解溫度的確定    4 小米糠中阿拉伯木聚糖的分離提取研究:http://www.aftnzs.live/shiping/20190416/32159.html
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