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鹽脅迫下菊芋糖代謝變化機制研究

時間:2019-06-30 08:04來源:畢業論文
鹽逆境使菊芋塊莖減產的內在原因。對7、8兩個月菊芋的可溶性總糖進行了測量,結果顯示不同濃度鹽脅迫下的菊芋塊莖、莖稈及葉片中可溶性糖和還原糖含量變化

摘要:本研究利用新一代高通量測序手段——轉錄組測序(RNA-Seq)技術,采用PE150測序策略,結合糖分在塊莖、莖稈和葉片中的積累與分配情況,以期闡明鹽脅迫下菊芋糖代謝變化和糖代謝相關基因的表達調控機制,進而說明鹽逆境使菊芋塊莖減產的內在原因。對7、8兩個月菊芋的可溶性總糖進行了測量,結果顯示不同濃度鹽脅迫下的菊芋塊莖、莖稈及葉片中可溶性糖和還原糖含量變化如下:(1)菊芋塊莖總糖含量在7月高鹽處理時含量最高,達到了847.05 mg/g;而還原糖含量則是7月低鹽環境下最高,為59.92 mg/g;(2)菊芋莖稈中總糖和還原糖含量的最高值分別出現在8月高鹽和7月低鹽環境下,分別為719.04 mg/g和76.52 mg/g;(3)菊芋葉片中總糖和還原糖含量的最高值則分別出現在8月低鹽和高鹽環境下,分別為185.45 mg/g和84.40 mg/g。轉錄組測序結果表明,共測得442902313 raw read pairs,經過質控,得到423231876 clean read pairs,并根據菊芋的差異組合,共鑒定出8496個差異基因。通過對差異基因的功能富集,分析發現了鹽脅迫下菊芋糖代謝相關基因的表達調控機制:(1)蔗糖合成酶參與了蔗糖的合成,后者在植物的生長發育、滲透調節和植物適應逆境的脅迫反應過程中都有舉足輕重的作用,實驗分析表明蔗糖合成酶的基因在菊芋低鹽和菊芋高鹽中均受脅迫誘導下調表達;(2)菊芋塊莖在低鹽環境下編碼己糖轉運蛋白 (hexose transporter) 的基因表現為下調,糖轉運蛋白可以通過調節糖的轉運來控制植物體內糖分的分配,當植物受到逆境脅迫時,糖轉運蛋白的活動受到抑制而減少,進而影響到植物的生理活動。36752
畢業論文關鍵詞:菊芋;鹽脅迫;高通量測序;轉錄因子;轉錄組分析
源¥自%六^^維*論-文+網=www.aftnzs.live

The research on the mechanism of Sugar metabolic changes of Helianthus tuberosus under salt stress
Abstract:This study uses a new generation of high-throughput sequencing method-the transcriptome sequencing (RNA-Seq) technology, adopting PE150 sequencing strategy, combining with the phenomenon about accumulation and distribution of soluble sugar and reducing sugar in the tube, stem and leaf, to clarify mechanism of expression and regulation of genes related to sugar metabolism under salt stress, then explain the internal causes of the Helianthus tuberosus’s lower production. The soluble sugar and reducing sugar was measured in July and August months, the results showed that the content of two sugars in tuber, stem and leaf are changed in different salt concentration change: (1) The total sugar content of the tuber is highest in high salt treatment in July, it's 847.05 mg/g. And the reducing sugar content is the highest in low-salt environment in July numbered 59.92 mg/g; (2) The peaks of total sugar and reducing sugar in the stalk are present in high salt in August and low salt in July, is 719.04 mg/g and 76.52 mg/g; (3) The highest levels of total sugar and reducing sugar in leaf was found in low salt and high salt in August, were 185.45 mg/g and 84.40 mg/g respectively. The transcriptome sequencing results show that there is 442902313 raw read pairs, and remained 423231876 clean read pairs after quality control, identified 8496 different genes finally. Analysis these different genes, the mechanism of expression and regulation of genes related to sugar metabolism under salt stress were found: (1) Sucrose played an important role in the growth and development of plants, osmotic regulation, cell metabolism and the stress response of plants to adapt to adversity. This sequence of processes was done by the sucrose synthesis (SuSy) . The genes that encode sucrose synthase (SuSy) , which were induced both under the high and low salt stress; (2) The distribution of sugar in plants could be controled through regulating the transport of sugar by hexose transporter. The activity of sugar transporters was reduced when plants were under stress , and then affected the physical activity of plants. The gene that encoded hexose transporter in low-salt environments had been shown to be down. 鹽脅迫下菊芋糖代謝變化機制研究:http://www.aftnzs.live/shengwu/20190630/35323.html
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