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環境微生物對大豆根瘤菌USDA110結瘤的影響

時間:2019-06-30 07:43來源:畢業論文
以219種大豆根瘤為材料,設置對照,并對處理組接種根瘤菌USDA110,提取兩組樣品根瘤DNA,分析各自的細菌群落,然后采用劃線法分離內生細菌。應用16S rDNA分析某些所具有的促生功能

摘要:豆科植物與根瘤菌通過聯合而形成固氮共生體,其最具代表性的特點是由指定的微共生體誘導得到根瘤或莖瘤。除卻根瘤菌外,在根瘤中也同樣存在非常多與根瘤菌共生固氮相關的內生細菌類群,而且根瘤菌與內生細菌一起存在于相同的根瘤內是非常常見的一種征象,屬于這類的內生細菌生于根瘤內,但不會使植物招致疾病,但與之相關的生態學作用還知道得非常少,本文以219種大豆根瘤為材料,設置對照,并對處理組接種根瘤菌USDA110,提取兩組樣品根瘤DNA,分析各自的細菌群落,然后采用劃線法分離內生細菌。應用16S rDNA分析某些所具有的促生功能。36748
畢業論文關鍵詞:大豆根瘤;內生細菌;16S rDNA;促生;匹配結瘤
The Effect to the USDA110 of Endogenous Bacteria from Root Nodule
Abstract:During the synaptic formation of nitrogen-fixing symbiont between legumes and rhizobia,the typical featureis the formation of root nodules and/or stem nodules induced by specific microsymbiont. Except for rhizobia,inside the root nodules there are various endogenous bacteria that have relation to rhizobial symbiotic, nitrogen fixation been isolated and the general coexistence of rhizobia with the bacteria in the same root nodule is ubiquitously objective phenomena. And existed in the root nodules were not able to induce the plants into morbidity. However very little of their ecological role have been aware of. This research took the 219 types of soybean nodules as samples,the control group was set up, and the experimental group was inoculated with the rhizobia USDA110, the DNA of the two samples was extracted and the bacterial community was analyzed .Then isolating endophytes by plate streaking. 16S rDNA sequence analysis were applied to explore the bacterias which have plant growth promoting characteristics. 源¥自%六^^維*論-文+網=www.aftnzs.live
Key words: soybean nodule;endophytic bacteria;16S rDNA;plant growth promotion;matching of nodulation
目  錄
摘要    3
關鍵詞    3
Abstract    3
Key words    3
引言    3
一  大豆根瘤內生細菌的分離及對USDA110結瘤的影響    5
1  材料與方法    5
1.1  供試材料    5
1.1.1  菌株    5
1.1.2  培養基及培養條件    5
1.1.3  大豆品種    6
1.2  實驗方法    10
1.2.1  種子催芽    10
1.2.2  土壤中結瘤實驗    10
1.2.3  DNA 的提取    11
1.2.4  挑選出16菌的16S DNA PCR擴增    11
1.1.5  內生細菌與USDA110的聯合接種    11
2  結果與分析    11
2.1   總16S rDNA分析    11
2.  1.  1細菌群落結構在目水平上旳變化    11
2.  1.  2 細菌群落結構在屬水平上的變化    13
2.  2  對分離的16個菌株的分析    14
2.  3  根瘤內生細菌對USDA110結瘤的影響    14
3  討論    15
二    USDA110與大豆品種匹配性的研究    16
1  材料與方法    16
1.1  供試材料    16
1.1.1  菌株    16
1.1.2  培養基及培養條件    16
1.1.3  大豆品種    16
1.2  實驗方法    16
源¥自%六^^維*論-文+網=www.aftnzs.live

1.2.1  種子催芽    16
1.2.2  花盆消毒    16 環境微生物對大豆根瘤菌USDA110結瘤的影響:http://www.aftnzs.live/shengwu/20190630/35319.html
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