畢業論文

打賞
當前位置: 畢業論文 > 生物論文 >

大豆離體葉片對斜紋夜蛾抗選性鑒定方法完善及應用

時間:2019-06-24 20:01來源:畢業論文
研究完善了離體葉片抗選性鑒定方法,利用打孔器將葉片截成直徑2.5cm的方形,將三組處理和對照的方形葉片放置于15cm口徑培養皿兩側,并接種三齡斜紋夜蛾幼蟲,危害18小時后進行數據

摘要:斜紋夜蛾是亞洲地區的主要食葉性害蟲。本研究完善了離體葉片抗選性鑒定方法,利用打孔器將葉片截成直徑2.5cm的方形,將三組處理和對照的方形葉片放置于15cm口徑培養皿兩側,并接種三齡斜紋夜蛾幼蟲,危害18小時后進行數據調查。選用9cm和15cm規格培養皿的F值分別為3.17和8.11(P<0.01),說明15cm相對于9cm規格的培養皿鑒定結果更為準確和可靠;接蟲危害14和18小時的F值分別為3.12和8.11(P<0.01),說明危害18小時更利于區分抗蟲和感蟲材料之間的抗選性差異。接蟲18小時,以南農1138-2作為對照品種,對IRSGP群體進行初篩,篩選出高抗和高感種質各10份;然后以欒川城關春黑豆和監利牛毛黃分別作為抗蟲和感蟲對照品種進行復篩,以驗證初篩結果。通過MLM方法對IRSGP群體進行關聯分析,共檢測到5個抗選性QTL。36605
畢業論文關鍵詞:大豆;斜紋夜蛾;抗選性;離體葉片
The improvement and application of detached leaflet evaluation method for antixenosis to common cutworm in soybeanStudent majoring in agronomy   
Abstract: Common cutworm is (CCW) a major leaf-feeding pest of soybean. In this study, the detached leaflet evaluation method for antixenosis to CCW was improved in soybean. In the detached leaflet evaluation method for antixenosis, leaflet was cut into a square with a diameter of 2.5 cm by punching bear, and the three groups of treated and control square leaflets were placed on both sides of the 15 cm diameter Petri dish, and the third-instar CCW larva were applied to investigate data after artificial infestation for 18 h. The F value of the 9cm and 15cm were respectively 3.17 and 8.11 (P<0.01), indicating the 15cm was more accuracy and stable than 9 cm. The F value of artificial infestation for 14 h and 18 h were respectively 3.12 and 8.11 (P<0.01), indicating artificial infestation for 18 h was helpful to distinguish the antixenosis difference between resistant and susceptible accessions. The IRSGP was firstly screened out 10 resistant and 10 susceptible accessions after artificial infestation for 18 h (the control variety is NN1138-2), and the secondary screening was used to verify the accuracy for first screening of results with the resistant and susceptible control varieties are respectively LCCGCHD and JLNMH. Five antixenosis QTLs were detected in IRSGP by MLM method of association mapping. 源¥自%六^^維*論-文+網=www.aftnzs.live
Key words: Soybean; Common cutworm; Antixenosis; Detached leaflet
目  錄     

摘要1
Abstract1
Key words 1
引言1
1 材料與方法2
1.1 試驗材料2
1.2 葉片來源3
1.3 斜紋夜蛾幼蟲的人工繁殖3
1.4設備和流程3
1.5 試驗設計4
1.6 數據分析5
1.7 全基因組關聯分析5
2 結果與分析5
 2.1 離體葉片抗選性鑒定方法的完善5
2.2 IRSGP群體的抗選性差異5
2.3 IRSGP群體的全基因組關聯分析7
3 討論7
致謝8
參考文獻8
大豆離體葉片對斜紋夜蛾抗選性鑒定方法的完善及應用
引言:大豆是世界上重要的油料作物之一,其籽粒中的高蛋白和油脂具有很大的利用價值[1]。斜紋夜蛾(Spodoptera litura Fabricius)屬鱗翅目夜蛾科,是暴食和雜食性強的一種食葉類害蟲,被危害的植物包括單子葉和雙子葉共計109科389種植物[2]。隨著氣候的變化,斜紋夜蛾種群數量在我國華東地區逐年升高,導致大豆產量下降,嚴重的甚至絕收[3]。害蟲的綜合治理主要包括化學物理生物等防治方法,其中化學和物理方法對環境造成的影響比較大,而生物防治具有環保和可持續化的特點[4]。隨著人們對無公害綠色食品需求及環境保護意識的日益增強,培育抗蟲品種成為控制蟲害、保護環境的重要途徑[5]。 大豆離體葉片對斜紋夜蛾抗選性鑒定方法完善及應用:http://www.aftnzs.live/shengwu/20190624/35124.html
------分隔線----------------------------
推薦內容
双色球走势图带连线