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酵母菌發酵生產γ-癸內酯的乳化體系構建及其對產物轉化的影響

時間:2019-06-02 15:44來源:畢業論文
對酵母菌以蓖麻油酸為底物發酵生產γ-癸內酯的乳化體系構建,及其對產物轉化的影響進行了研究。此發酵體系為水包油體系,油相底物難以進入酵母,阻礙酵母對底物蓖麻油酸的利用

摘要:γ-癸內酯(GDL)是一種內酯類香味物質,主要用于配制奶油、桃子、柑橘和椰子型等香精。本文對酵母菌以蓖麻油酸為底物發酵生產γ-癸內酯的乳化體系構建,及其對產物轉化的影響進行了研究。此發酵體系為水包油體系,油相底物難以進入酵母,阻礙酵母對底物蓖麻油酸的利用與轉化,故本實驗通過向培養基中加入不同的表面活性劑,助表面活性劑,以及進行表面活性劑的復配從而使發酵液乳化,研究不同情況下底物粒徑的變化與γ-癸內酯產量間的關系;研究底物粒徑與酵母對底物的利用、γ-癸內酯生產間的關系。本課題實驗結果表明:單獨使用表面活性劑吐溫 20的乳化效果及生產的γ-癸內酯產量最佳,在底物與吐溫 20加入的濃度配比為10:1,助表面活性劑甘油與吐溫 20加入的濃度配比為1:2時,產物γ-癸內酯的轉化情況有最為明顯的提高,可從0.34g/L提升至0.53g/L,γ-癸內酯的產量增加了55.9%。產物γ-癸內酯的轉化情況與底物的粒徑及分散系數,即與酵母菌發酵生產γ-癸內酯的乳化體系的乳化情況有一定的關聯。35937
畢業論文關鍵詞: γ-癸內酯;乳化體系;蓖麻油酸
The construction of the emulsifying system of producing  γ- decalactone by fermentation from yeast and its impact on the transformation of product
Abstract:γ-decalactone (GDL) is a fragrance substance lactones, mainly used for the preparation of flavor of cream, peach, citrus and coconut, etc. In this paper, the construction of the emulsifying system of producing γ-decalactone by fermentation from yeast and its impact on the transformation of product were investigated. This fermentation system is O/W, the oil phase substrate is difficult to enter the yeast, it block the utilization and conversion of substrate ricinoleic acid by yeast, so the relationship between different size of substrate and the productivity of γ-decalactone in different situations, the relationship between the size of substrate and utilization of substrate by yeast, and the relationship between the size of substrate and the productivity of γ-decalactone were investigated by adding different surfactants and cosurfactant to the medium, and compounding the surfactants to emulsify the fermentation liquor in this report. The result shows: The best effect on emulsifying and the productivity of γ-decalactone is using Tween 20 alone, and when it is added with the substrate: Tween 20=10:1, cosurfactant glycerol: Tween 20=1:2, the transformation of product γ-decalactone is increased obviously, from 0.34g/L to 0.53g/L, production of γ-decalactone increased by 55.9%. And there has some relative between the transformation of γ-decalactone and substrate size and coefficient of dispersion. In other words, there has some relative between the transformation of γ-decalactone and yeast fermentation production of emulsifying γ-decalactone emulsion system.
源¥自%六^^維*論-文+網=www.aftnzs.live

KeyWords: γ- decalactone;emulsifying system;ricinoleic acid
目  錄
1緒論    1
1.1 本課題在國內外的發展概況及存在的問題    1
1.1.1關于發酵生產γ-癸內酯    1
1.1.2關于乳化體系的構建    2
1.2 本課題的研究目的    4
1.3 本課題的研究意義    4
1.4 本課題的研究內容    5
2實驗材料和方法    5
2.1 實驗材料    5
2.1.1 試劑    5
2.1.2 試驗用菌種    5
2.1.3 發酵培養基    5
2.1.4 儀器設備    5
2.2 實驗方法    6
2.2.1 GDL的氣相檢測方法及標準曲線的制作    6 酵母菌發酵生產γ-癸內酯的乳化體系構建及其對產物轉化的影響:http://www.aftnzs.live/shengwu/20190602/34159.html
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