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雙水相體系從發酵液中提取γ-聚谷氨酸

時間:2019-06-02 14:40來源:畢業論文
針對γ-PGA在提取純化環節存在的問題,嘗試采用PEG/(NH4)2SO4雙水相體系從發酵液中提取γ-PGA。通過改變兩相類型、質量配比和體系條件,確定最佳提取工藝,PEG1000質量分數為23%

摘要:γ-聚谷氨酸(γ-Poly glutamic acid,簡稱γ-PGA)是一種由微生物合成的胞外水溶性單體氨基酸聚合物,具有優良的保濕性、生物相容性和可降解性。本課題針對γ-PGA在提取純化環節存在的問題,嘗試采用PEG/(NH4)2SO4雙水相體系從發酵液中提取γ-PGA。通過改變兩相類型、質量配比和體系條件,確定最佳提取工藝,PEG1000質量分數為23%,硫酸銨質量分數為12.5%,發酵液質量分數為10%,發酵液pH值為8,體系溫度為25℃時,最大分配系數為2.31,提取率為78.02%。35927
畢業論文關鍵詞:γ-聚谷氨酸;提取;雙水相
Extraction of γ-Polyglutamic acid from fermentation liquid by using aqueous two-phase
Abstract γ-Poly glutamic acid(γ-PGA) is a kind of microbe exocellular watersoluble monomer amino acid high polymer. γ-PGA is moisturizing, biocompatibility and biodegradable. Therefore, γ-PGA and its derivatives have been of interest in a broad range of industrial fields such as cosmetics, agriculture, medicine and food. At present the purification and extraction process is the biggest factor affecting the industrialization of γ-PGA. We attempted the PEG/ammonium sulfate two aqueous phases system to extract γ-PGA in fermentation liquid. And the best extraction process was established by changing the type and mass ratio of the two-phase as well as the condition of the system.When PEG1000 mass fraction was 23%, (NH4)2SO4 mass fraction was 12.5%, fermentation liquid mass fraction was 10%.Under this condition, the pH value of the fermentation liquid was 8, the system temperature was 25°C. The maximum of the distribution coefficient K and the extraction rate of Y were respectively. The distribution coefficient K is 2.31, and the extraction rate of Y is 78.02%.
Key words γ-Polyglutamic acid;aqueous two-phase;Extraction 源¥自%六^^維*論-文+網=www.aftnzs.live
目 錄
1緒論    1
    1.1 γ-PGA的簡介[1~3]    1
1.2 γ-PGA的性質    1
1.2.1 吸水特性    1
1.2.2 生物可降解性    2
1.2.3 γ-PGA的水解特性    2
1.3 γ-PGA的應用[21]    2
1.3.1 γ-PGA在農業中的應用    2
1.3.2 γ-PGA在日用品中的應用    3
1.3.3 γ-PGA在醫藥中的應用    3
1.3.5 γ-PGA在食品工業中的應用    4
1.4 國內外發酵產γ-PGA的提取工藝研究現狀[7~10]    4
1.4.1發酵液預處理    5
1.4.2分離純化    5
1.5 研究目的和意義    6
1.5.1 研究目的    6
1.5.2 研究意義    6
1.5.3 研究任務    6
2.材料和方法    7
2.1材料    7
2.1.1 菌種    7
2.1.2 主要實驗試劑    7
2.1.3 主要儀器和設備    7
2.2 發酵液的制備    8
2.2.1 培養基成分及培養條件    8
2.2.2 發酵液預處理    9
2.3 實驗方案    9
2.3.1 實驗原理    9
2.3.2 實驗方案    9
3 結果與討論    10
3.1不同質量分數的硫酸銨和PEG對γ-PGA的水解的影響及吸收峰的影響。    10 源¥自%六^^維*論-文+網=www.aftnzs.live
3.2硫酸銨和PEG自身對吸收峰的影響    10
3.3標準曲線的繪制    10
3.4單因素實驗的條件優化    11
3.4.1 PEG質量分數對分配系數及提取率的影響    11
3.4.2 硫酸銨質量分數對分配系數及提取率的影響    12 雙水相體系從發酵液中提取γ-聚谷氨酸:http://www.aftnzs.live/shengwu/20190602/34144.html
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