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水稻與高粱NBS-LRR家族部分蛋白的序列與快速進化

時間:2019-05-27 13:16來源:畢業論文
利用生物信息學手段第一次系統而全面地分析了水稻與高粱NBS-LRR蛋白中14 種關鍵蛋白基因的序列與進化特征

摘要:水稻和高粱是擁有高質量的生物作物生產的潛力和工業價值的糧食作物家族。生物能源植物變得容易直接受到各種生物壓力,這影響糧食作物的質量和容量。在植物物種中至今沒有報告存在分析核苷酸綁定NBS-LRR蛋白以及轉錄因子的防御反應。在水稻和高粱的物種的防御反應相關的轉錄因子中,全基因組和轉錄組的分析確定新NBS-LRR蛋白。所確定的NBS-LRR和防御反應基因轉錄因子映射到各自的基因組。常見的和獨特的NBS-LRR基因和防御相關的轉因子在植物物種中被確定。植物生長是由生物和非生物壓力r基因表現出反抗的疾病和植物的病原體嚴重影響的。從進化和基因組序列分布這方面來說,核苷酸結合位點和亮氨酸重復(NBS-LRR)類豐富的r基因是研究最全面的。對生物和非生物壓力的輕微反應一直在糧食作物的栽培中被觀察。本研究利用生物信息學手段第一次系統而全面地分析了水稻與高粱NBS-LRR蛋白中14 種關鍵蛋白基因的序列與進化特征。序列分析顯示這樣的一個結果:同一類NBS-LRR蛋白基因結構相似性非常大,其中大多數基因的外顯子個數比較保守;同一類NBS-LRR蛋白基因由相似的結構特征和蛋白保守基序組成,其中LOC_Os11g37050和LOC_Os11g38520具有相似的結構特征和蛋白保守基序。由模型分析可知,我們檢測到正選擇位點。35776
畢業論文關鍵詞:NBS-LRR;水稻;高粱;結構域;正選擇位點
Sequence and Rapid Evolution of the NBS-LRR Subfamily Genes in Rice and Sorghum
Abstract: Rice and sorghum are biological potential of crop production and high quality industrial value of food crops to family. Bioenergy plants easier by various biological pressure directly, the impact on the quality of food crops and capacity. The NBS-LRR proteins have been reported in the model plants, but the NBS-LRR proteins remain unclear in rice and sorghum. Defense responses in rice and sorghum species associated with transcription factors, the whole genome and transcriptome analysis to determine new NBS LRR protein. The identified NBS LRR and defense response genes transcription factors mapped to the respective genomes. Common and unique NBS LRR gene and defense related transcription factor in plant species were identified. Plant growth is made up of biological and non-biological stress r genes showed resistance of disease and plant pathogens. In terms of the evolution and genome sequence distribution, nucleotide binding site and leucine repeated (NBS LRR) class rich r gene is the most comprehensive. Mild reaction to the biological and non-biological stress has been observed in the course of cultivation of food crops. Based on bioinformatics methods, we study the NBS-LRR subfamily proteins in rice and sorghum for the first time. Sequence analysis showed that such a result: the same kind of NBS LRR protein gene structure similarity with large, including number of  gene exon is conservative; The same kind of NBS-LRR protein gene by conservative motif similar structural characteristics and protein composition, the LOC_Os11g37050 and LOC_Os11g38520 have similar structural characteristics and protein conservative motif. Known from the analysis of the model, we detect positively selected sites.
源¥自%六^^維*論-文+網=www.aftnzs.live

Keywords: NBS-LRR, Rice, Sorghum, Domain, Positively selected sites
目    錄
摘要    1
引言    2
1 數據與方法    3
1.1 數據來源與序列下載    3
1.2 基因結構特征鑒定    3
1.3 蛋白序列特征分析    3
1.4 蛋白選擇壓力檢測    3
1.5 基因表達譜分析    4
2 結果與分析    4
2.1 水稻與高粱NBS-LRR家族成員的鑒定    4
2.2 水稻與高粱NBS-LRR家族成員基因的結構特征    5 水稻與高粱NBS-LRR家族部分蛋白的序列與快速進化:http://www.aftnzs.live/shengwu/20190527/33883.html
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