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植物幾丁質酶的生物信息學分析

時間:2019-05-21 13:03來源:畢業論文
選擇的六類植物的幾丁質酶蛋白序列長度和等電點差異不大,這幾種蛋白序列均存在5-8個紊亂區,它們都屬于球蛋白,且序列中20中常見氨基酸組成接近;六種植物幾丁質酶的二級結構均

摘 要:幾丁質酶(EC3.2.1.14)是一類在于植物、動物、微生物中的能降解幾丁質的糖苷酶,與植物體內抗真菌性的防御系統有關,是一種重要的病程相關蛋白。本文通過NCBI數據庫已登錄的小麥(Triticum aestivum AAR11388.1)、高粱(Sorghum bicolor XP-002488863.1)、水稻(Oryza sativa japonica group NP_001050373.2)、蓖麻(Ricinus communis XP_002525743.1)、擬南芥(Ar-abidopsis thaliana XP_00284907.1)、玉米(Zea  mays AAT40016.1) 等植物的蛋白序列進行查詢下載,分別對其進行理化性質分析、亞細胞定位、跨膜區、磷酸化位點分析、同源比對、構建進化樹以及二、三級結構預測等一系列生物信息學分析。結果表明,這些蛋白均存在信號肽序列,均為分泌性蛋白,定位于細胞液中,含有多個不同的磷酸化位點;選擇的六類植物的幾丁質酶蛋白序列長度和等電點差異不大,這幾種蛋白序列均存在5-8個紊亂區,它們都屬于球蛋白,且序列中20中常見氨基酸組成接近;六種植物幾丁質酶的二級結構均以無規則卷曲為主。通過進化樹的構建可知,通過構建進化樹得知,這幾種植物相似性較高,進化距離較近。這些結果為幾丁質酶的進一步深入研究提供了理論基礎。35625
畢業論文關鍵詞:植物;幾丁質酶;生物信息學
Bio-informatics Analysis of Plant Chitinase
Abstract:Chitinase (EC3.2.1.14) is a class that plants, animals, microorganisms can degrade chitin (N- acetylglucosamine linear oligomers) glycosidases. Its resistance to fungi and plants related to defense systems, is an important pathogenesis-related proteins. We queryed and download Triticum aestivum ( AAR11388.1)、Sorghum bicolor( XP-002488863.1)、Oryza sativa japonica group (NP_001050373.2)、Ricinus communis (XP_002525743.1)、Arabidopsis ( XP_00284907.1)、Zea mays (AAT40016.1)  protein sequence of the chitinase from the NCBI database. We are analyzed their basic physical and chemical properties, subcellular localization, transmembrane region, homology comparison and phylogenetic tree, as well as secondary and tertiary structure prediction and a series of bioinformatic analysis to understand its structure and function related information. These results indicate that the signal peptide sequences of these proteins are present, are secreted proteins, located in the cytosol, it contains a number of different phosphorylation sites; chitinase protein sequence length and isoelectric selection of six plants point is insignificant, these types of protein sequences are present disorder zones 5-8, they all belong to globulin, and the sequence of 20 amino acids commonly found close; the secondary structure of the six plants of chitinase are no rules curly-based. Rhodopseudomonas palustriss because they had close evolutionary distance. Seen through the evolutionary tree constructed by constructing evolutionary trees that, these types of higher plant similarity, close evolutionary distance. All results provide a theoretical basis for further research. 源¥自%六^^維*論-文+網=www.aftnzs.live
Keywords: plant; chitinase; Bioinformatics analysis
目    錄
摘 要    1
引 言    2
1 材料和方法    3
   1.1材料來源    3
   1.2 生物信息學分析軟件    4
2 結果與分析    5
2.1生物信息學分析    5
2.1.1 基本理化性質分析    5
2.1.2幾丁質酶跨膜區預測    7
2.1.3幾丁質酶的信號肽分析    8
2.1.4 幾丁質酶的亞細胞定位    8
2.1.5幾丁質酶磷酸化分析    9
2.2 幾種蛋白序列的同源性比對與同源進化樹的構建    9
2.3 二級結構、蛋白紊亂區、球蛋白區預測    11
2.3.1蛋白質二級結構預測    11 植物幾丁質酶的生物信息學分析:http://www.aftnzs.live/shengwu/20190521/33658.html
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