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吡蟲啉在玉米中的殘留檢測方法研究

時間:2019-05-17 19:30來源:畢業論文
建立了高效液相色譜檢測土壤和玉米中吡蟲啉殘留的方法。土壤、玉米籽粒和植株樣品分別采用乙腈、二氯甲烷提取, SPE固相萃取柱凈化, 通過高效液相色譜紫外檢測器進行檢測。在0.0

摘要:本文建立了高效液相色譜檢測土壤和玉米中吡蟲啉殘留的方法。土壤、玉米籽粒和植株樣品分別采用乙腈、二氯甲烷提取, SPE固相萃取柱凈化, 通過高效液相色譜紫外檢測器進行檢測。在0.02~10 mg/L范圍內,吡蟲啉濃度與色譜峰面積呈良好線性關系, 相關系數為1。其最小檢出量為0.06ng,最低檢出濃度為0.01 mg/kg。在添加濃度為0.02 ~ 1 mg/kg范圍內,玉米籽粒中平均回收率為85.5 ~ 98.9%;玉米植株中平均回收率為 86.5 ~ 93.1%;添加濃度為 0.01 ~ 1 mg/kg范圍內,土壤中平均回收率為 90.7 ~ 94.8%。該方法的準確度和精密度符合農藥殘留分析要求,操作簡便快速, 節約溶劑, 可為吡蟲啉在玉米中的殘留分析提供技術依據。35413
畢業論文關鍵詞:吡蟲啉;玉米;農藥殘留;高效液相色譜
Study on the residue detection of Imidacloprid in Maize
Abstract: An efficient method was developed for the quantification of imidacloprid residues in soil and maize by high performance liquid chromatography (HPLC). Soil and plant samples were extracted with acetonitrile, dichloromethane, SPE solid phase extraction column and detected by high performance liquid chromatography (UV) detector. In the range of 0.02~10 mg/L, the concentration of imidacloprid was linear with the peak area of the chromatogram, and the correlation coefficient was 1. The limit of detection(LOD) was 0.06 ng and the limit of quantitation(LOQ) was 0.01 mg/kg. The average recoveries of maize were range from 85.5 to 98.9% at spiked of 0.02~1 mg/kg, and the recoveries of maize plant were 86.5~93.1%. For the soil sample, the recoveries were in the range of 90.7~94.8% at the spiked of 0.01~1 mg/kg. The accuracy and precision of this method are in accordance with the requirements of pesticide residue analysis. The method is simple, rapid to operate and lower consumption of solvents, which can provide technical basis for the analysis of imidacloprid in maize and soil.
源`自*六)維[論*文'網www.aftnzs.live

Key words: Imidacloprid; High performance liquid chromatography (HPLC). Maize; Residue analysis
目  錄
摘要    1
關鍵詞    1
Abstract    1
Key words    1
1 選題背景    1
1.1 農藥殘留    1
1.2 吡蟲啉的殘留分析方法研究進展    2
2 材料與方法    3
2.1儀器設備    3
2.2試劑    3
2.3色譜條件    3
2.4 吡蟲啉標準溶液配制    3
2.5 建立吡蟲啉標準曲線    3
2.6樣品前處理    3
2.6.1 樣品提取    3
2.6.2 樣品凈化    3   
2.7 添加回收實驗    4
3 結果與分析    4
3.1 建立標準曲線    4
3.2 添加回收率與相對標準偏差    5
4 討論與結論    5
4.1 儀器參數選擇    5
4.2 流動相對色譜分離的影響    6
4.3 樣品先處理優化    6
4.3.1  提取溶劑的選擇    6
4.3.2  凈化方法的選擇    7
4.4結論    7
致謝    7
參考文獻    7
吡蟲啉在玉米中的殘留檢測方法研究
1    選題背景
1.1 農藥殘留
農藥在人類的生產生活中有著重要的意義,特別是在減少勞動力需求,防治植物病蟲害,培育新的作物品種等方面起到了關鍵的作用,為人類社會的經濟效益和社會效益提供巨大發展動力。初期的農藥以快速殺死和消滅有害生物為根本,產品毒性相對較高,加之人們環保意識相對薄弱,對農藥的相關理論知識不了解造成使用不合理,導致了農藥殘留超標等問題的出現。 吡蟲啉在玉米中的殘留檢測方法研究:http://www.aftnzs.live/shengwu/20190517/33352.html
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