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水稻秸稈為原料的纖維素酶發酵條件優化

時間:2019-04-19 21:22來源:畢業論文
論文主要是用單因子和正交試驗來探討黑曲霉產纖維素酶受培養基組成及發酵條件的影響,主要從含水量、水稻與豆粉比、溫度、發酵天數、pH、硫酸銨、接種量等來探究對纖維素酶酶活

摘要:全球目前面臨著環境污染的加劇以及石油危機,越來越多的國家開始利用乙醇作為替代燃料。我國作為農業大國,可以充分利用農作物秸稈發酵生產乙醇,以解決我國的能源問題,然而農作物秸稈生產乙醇由于分解困難而導致產量低但生產成本高,于是我們應該提升產量以減少乙醇的生產費用。本論文旨在通過提高纖維素酶的酶活,將秸稈中的纖維素轉化成可發酵性糖。本論文主要是用單因子和正交試驗來探討黑曲霉產纖維素酶受培養基組成及發酵條件的影響,主要從含水量、水稻與豆粉比、溫度、發酵天數、pH、硫酸銨、接種量等來探究對纖維素酶酶活的影響。通過實驗得出最優培養基的成份和含量:水稻2.4 g,豆粉0.6 g,水70%,硫酸銨4%,磷酸二氫鉀0.2%,硫酸鎂0.2%,pH 7,接種量5×106。最優的培養條件為:培養溫度41℃,發酵天數6天。通過對發酵條件的優化,黑曲霉產纖維素酶的酶活由374.55 U/g提高到851.90 U/g。34672
畢業論文關鍵詞:纖維素酶、黑曲霉、發酵條件、正交試驗
Optimization of cellulase fermentation conditions of rice straw as raw material
Abstract: The world is facing the increasing environmental pollution and oil crisis, More and more countries began using ethanol as a substitute fuel. China is an agricultural country, The energy problem can be solved by ethanol fermentation using crop straw, However, It’s ethanol yield is low and cost is high to produce ethanol using crop straw, So it’s important to improve the yield and reduce production cost. This paper is aimed at improving the activity of cellulose which can transform cellulose to fermentable sugar. This experiment is mainly through single factor and orthogonal experiment to study the effects of medium composition and culture conditions on cellulase production by Aspergillus niger, Mainly on water content, ratio of rice to flour, temperature, fermentation time, pH, ammonium sulfate, inoculation amount effect on the activity of cellulose. In order to obtain the optimal medium recipe: rice 2.4 g, soybean flour 0.6 g, water 70%, 4% (NH4)2SO4, 0.2% KH2PO4, 0.2% MgSO4, pH 7, inoculation 5×106. The optimal culture conditions: culture temperature 41℃, fermented for 6 days. Aspergillus niger cellulase production was increased from 374.89 U/g to 906.67 U/g through the optimization of cellulose fermentation conditions.
源¥自%六^^維*論-文+網=www.aftnzs.live

Keywords: cellulase, Aspergillus niger, fermentation conditions, orthogonal experiment
目錄
引言    1
1.材料和方法    2
1.1材料    2
1.1.1菌種    2
1.1.2培養基    2
1.1.3試劑及配制    2
1.1.4儀器設備    2
1.2實驗方法    2
1.2.1菌種復蘇    2
1.2.2配制基礎產酶培養基    3
1.2.3接種以及固體發酵    3
1.2.4葡萄糖標準曲線的制作    3
1.2.5粗酶液的制取    3
1.2.6酶活力的測定    3
1.2.7酶活力的計算    3
2.結果與分析    4
2.1葡萄糖標準曲線    4
2.2培養基及培養條件的優化結果    4
2.2.1水稻與豆粉比對產纖維素酶的影響    4
2.2.2硫酸銨對產纖維素酶的影響    5
2.2.3接種量對產纖維素酶的影響    6
2.2.4發酵天數對產纖維素酶的影響    6
2.2.5含水量對產纖維素酶的影響    7
2.2.6 pH值對產纖維素酶的影響    8
2.2.7溫度對產纖維素酶的影響    8
2.3正交試驗優化培養基    9
2.4正交試驗結果與分析    9 水稻秸稈為原料的纖維素酶發酵條件優化:http://www.aftnzs.live/shengwu/20190419/32261.html
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