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降壓草中活性物質提取+文獻綜述

時間:2019-04-16 22:05來源:畢業論文
降壓草是多年生宿根草本,全株肉質,分枝性強,它的葉和桿含有總黃酮類化合物,具有研究價值。本課題中是運用有機溶劑萃取法提取降壓草中總黃酮,并改良實驗裝置和優化提取降

摘要:降壓草是多年生宿根草本,全株肉質,分枝性強,它的葉和桿含有總黃酮類化合物,具有研究價值。本課題中是運用有機溶劑萃取法提取降壓草中總黃酮,并改良實驗裝置和優化提取降壓草中總黃酮的工藝條件,還對所得提取液進行了純化,最后是對降壓草做活性成分分析及化學鑒定。結果表明有機溶劑(乙醇)提取降壓草樣品總黃酮的最佳工藝條件是:乙醇濃度95%,料液比1∶40 g/ml,提取時間1h;此條件下黃酮含有率為 4.7%。通過液相色譜法分析純化后的降壓草提取物,得出提取物中的確時含有總黃酮。通過鄰苯三酚法和DPPH法驗證了降壓草總黃酮具有顯著抗氧化性。34633
畢業論文關鍵詞:有機溶劑提取法,總黃酮;降壓草;抗氧化性
Extraction of Active Substance in Buck grasses
Abstract:Buck grass is a perennial herbaceous perennial, whole plant fleshy, strong branches, its leaves and stem contains flavonoid compounds, with research value. Using the organic solvent extraction of total flavonoids in grass buck, process conditions and improved experimental device and to optimize the extraction of licorice flavonoids, extract of total flavonoids were purified finally to buck grass active ingredient analysis and chemical identification. Results showed that extraction of organic solvent (ethanol) grass buck of total flavonoids of the sample, the optimum technological conditions are: 95% ethanol concentration, solid-liquid ratio of 1: 40 g / ml, extraction time was 1 hour; under the conditions of the flavonoids extraction rate was 4.7%. The extracts of the extracts were purified by HPLC, and the total flavonoids were contained in the extract.. The antioxidant activity of total flavonoids in the grass was determined by three phenol method and DPPH method.. The results showed that flavonoids in the extract of the antihypertensive herb had certain antioxidant activity.
源¥自%六^^維*論-文+網=www.aftnzs.live

KeyWords:Organic solvent extraction,total flavonids,buck grass,oxidation resistance
目錄
1 緒論    1
1.1 降壓草概述    1
1.2黃酮類化合物概述     2
  1.2.1 黃酮類化合物的結構     2
  1.2.2 黃酮類化合物的抗氧化性     3
  1.2.3黃酮類化合物提取的國內外研究     4
1.3本課題的研究內容、目的及意義     5
2 材料和方法    6
2.1 實驗材料與器材     6
2.1.1實驗材料     6
2.1.2 主要儀器    6
2.1.3 主要試劑    6
2.2 分析方法    6
2.2.1液相色譜分析提取液      6
2.2.2 總黃酮的抗氧化實驗    7
  2.3 實驗方法    9
2.3.1 探究影響降壓草中總黃酮提取的因素    9
2.3.2 優化提取降壓草總黃酮的工藝條件    9
2.3.3總黃酮提取率的計算    10
2.3.4液相色譜法分析    10
2.3.5最優條件下總黃酮的抗氧化性    11
2.3.6 活性物質的有機溶劑的提取工藝    11
3 結果與討論    11
3.1 探究影響降壓草中總黃酮提取的因素    12
3.1.1不同的提取劑     12
3.1.2降壓草的不同部位      13
3.1.3降壓草的干濕狀況     13
3.1.4提取液的濃度     13
3.1.5不同的料液比      14
    3.1.6提取時間     14 源¥自%六^^維*論-文+網=www.aftnzs.live
3.2 優化提取降壓草總黃酮的工藝條件    15
  3.2.1三水平三因素表    15 降壓草中活性物質提取+文獻綜述:http://www.aftnzs.live/shengwu/20190416/32215.html
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