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多胺和丁二酸復合劑對滲透脅迫下小麥幼苗的抗氧化代謝和光合特性的調控效應

時間:2019-04-14 13:36來源:畢業論文
小麥幼苗在滲透脅迫條件下,超氧化物歧化酶(SOD)、過氧化物酶(POD)和過氧化氫酶(CAT)的活性隨著滲透脅迫時間的延長,其活性逐漸降低,光合特性指標 (凈光合速率(Pn)、氣孔導

摘 要:為了探索多胺和丁二酸復合劑提高小麥幼苗抗旱性的機理,本實驗以周麥22為實驗材料,采用沙培的方式,探究小麥幼苗在滲透脅迫下,多胺和丁二酸復合劑對小麥幼苗的抗氧化代謝和光合特性的影響。實驗結果表明:小麥幼苗在滲透脅迫條件下,超氧化物歧化酶(SOD)、過氧化物酶(POD)和過氧化氫酶(CAT)的活性隨著滲透脅迫時間的延長,其活性逐漸降低,光合特性指標 (凈光合速率(Pn)、氣孔導度(Cs)、胞間CO2濃度(Ci)和蒸騰速率(Tr))降低, 而丙二醛(MDA)含量和超氧陰離子產生速率不斷的增加;外源多胺、丁二酸、多胺和丁二酸復合劑處理后,小麥幼苗的抗氧化代謝酶(SOD、POD、CAT)活性上升,MDA含量和超氧陰離子產生速率下降,光合指標上升,并且外源施加多胺及丁二酸復合劑效果顯著。這些實驗結果暗示多胺、丁二酸、多胺和丁二酸復合劑能通過提高滲透脅迫下小麥幼苗的抗氧化代謝酶活性和光合效率緩解滲透脅迫對小麥幼苗的傷害。34527
畢業論文關鍵詞:小麥幼苗;滲透脅迫;多胺和丁二酸復合劑;抗氧化代謝;光合特性
Regulation Effects of Polyamine and Succinate on the Antioxidant Metabolism and Photosynthetic Characters of Wheat Seedling under Osmotic Stress
Abstract:To explore the mechanism that polyamine and succinate improved the the drought resistance of wheat seeding, Zhoumai 22 was used as the material and sand culture was used in this experiment. The regulating effects that polyamine and succinate on the antioxidant metabolism and photosynthetic characters of wheat seeding under osmotic stress were studied . The experimental results showed that the activities of the superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and the photosynthetic characteristic index (Pn, Ci, Cond, Tr) decreased gradually with the days going on under osmotic stress, and the content of the MDA and the superoxide anion (O2•-)generation rate increased gradually; After the treatment of exogenous polyamine, succinate and their compound, the activities of SOD, POD, CAT and the photosynthetic characteristic index (Pn, Ci, Cond, Tr) increased, and the content of the MDA and O2•- generation rate decreased, and the effect of their compound was better. The results suggested exogenous polyamine, succinateand their compound could relieved the injuries of osmotic stress on wheat seedling via improving the anti-oxidant metabolic enzymes and photosynthetic efficiency of wheat seedlings.
源¥自%六^^維*論-文+網=www.aftnzs.live

Key words: The wheat seeding; Osmotic stress; Polyamine and Succinate compound; Antioxidant metabolism; Photosynthetic characteristics
 目    錄

摘 要    1
Abstract    1
引言    2
1材料與方法    3
1.1材料    3
1.2試劑    3
1.3材料培養與處理    3
1.4 測定內容與方法    4
1.4.1小麥幼苗葉片抗氧化物酶(SOD、POD、CAT)活性的測定    4
1.4.2小麥幼苗葉片丙二醛(MDA)、超氧陰離子產生速率的測定    5
1.4.3小麥幼苗葉片凈光合速率、胞間CO2濃度、氣孔導度、蒸騰速率的測定    5
2結果與分析    5
2.1 多胺、丁二酸、多胺及丁二酸復合劑滲透脅迫下小麥幼苗抗氧化代謝酶活性的影響    5
2.2 多胺、丁二酸、多胺及丁二酸復合劑滲透脅迫下小麥幼苗丙二醛、超氧陰離子含量的影響    7
2.3 多胺、丁二酸、多胺及丁二酸復合劑滲透脅迫下小麥幼苗葉片光合特性指標的影響    8
3結論與討論    10
參考文獻    11
致謝    14 多胺和丁二酸復合劑對滲透脅迫下小麥幼苗的抗氧化代謝和光合特性的調控效應:http://www.aftnzs.live/shengwu/20190414/32050.html
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