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鈷對集胞藻6803生長的影響研究

時間:2019-04-14 13:34來源:畢業論文
以集胞藻6803為材料,研究了不同濃度的Co2+處理對藻細胞生長的影響。結果表明:Co2+處理對集胞藻6803的生長及光合色素含量、蛋白質圖譜等都有顯著影響。當Co2+濃度為5μM時

摘  要:鈷是一種重金屬元素,是生物體內維生素B12的組成部分。然而,在冶金、顏料、陶瓷釉料等工業生產過程中,大量的鈷被排放至水體中。當鈷離子的濃度超過一定的范圍時,便會對生物造成相應的脅迫傷害。水體中的各種藻類、原生生物是包括鈷在內的各種重金屬脅迫首當其沖的受害者,而且重金屬會隨著食物鏈而逐級富集,對各級消費者尤其是人類的健康造成巨大的威脅。在水污染愈來愈嚴重的今天,實時監測水體中各種重金屬等污染物的濃度,研究典型水體生物對重金屬脅迫的生理響應及分子機制,通過物理化學或生物吸附的方法減少重金屬含量及其毒性效應,是亟待解決的問題。集胞藻6803是一種淡水生單細胞藍藻,因其個體微小、對各種脅迫響應迅速、環境適應性強、便于進行遺傳操作和生理指標測定等特點,是研究脅迫響應的理想材料。本研究以集胞藻6803為材料,研究了不同濃度的Co2+處理對藻細胞生長的影響。結果表明:Co2+處理對集胞藻6803的生長及光合色素含量、蛋白質圖譜等都有顯著影響。當Co2+濃度為5µM時,集胞藻6803的生長明顯受到抑制,同時藻細胞的葉綠素含量、類胡蘿卜素含量和蛋白含量等均顯著降低;當Co2+濃度為30µM時,集胞藻6803的生長完全被抑制,藻細胞基本停止生長甚至死亡。34525
畢業論文關鍵詞:鈷;集胞藻6803;生長;光合色素;蛋白質
Effects of cobalt on the growth of Synechocystis sp. PCC6803 源¥自%六^^維*論-文+網=www.aftnzs.live
Abstract: Cobalt is one of the heavy metal elements; it is an important component of vitamin B12 in vivo. However, in the process of industrial production such as metallurgy, dyestuff, ceramic glaze and so on, cobalt was largely discharged into water body, causing corresponding injure to variety of life in case its concentration is beyond a certain range. Algae and quite a lot of protista underwater is the first victim of various heavy metals including cobalt. Besides, heavy metal could be accumulated and enriched in the food chain, giving rise to great threat to consumers at all levels particular human health. Currently, water pollution is increasingly severe, and these subjects are urgently to be solved, for instance, monitoring the situation of heavy metal pollution in the water, study on the effects and molecular mechanism of heavy metal on the growth of typical aquatic organisms, reducing the content and alleviating the toxic effects of heavy metals by means of physicochemical and biological adsorption. Synechocystis sp. PCC6803 is one of the unicellular limnetic cyanobacteria which possess numerous advantages: small inpiduals, quick response to various stresses, well-adapted to a variety of environments and convenient for genetic manipulation, and these characteristics make Synechocystis one of the model organisms in study of stress responses. In the present research, effect of different concentration of cobalt on the growth of Synechocystis sp. PCC6803 was studied. The results showed that cobalt treatment impacts the growth, photosynthetic pigments contents as well as the protein profile of Synechocystis cells significantly. Specifically, when cells were cultured in the medium containing 5µM Co2+, the growth was retarded and the contents of chlorophyll, carotenoid and protein were reduced almost by half. While, 30µM Co2+ almost totally inhibited cell growth and even to die.
Keywords: Cobalt; Synechocystis sp. PCC6803; Growth; Photosynthetic pigments; Protein
目    錄 源¥自%六^^維*論-文+網=www.aftnzs.live

摘  要    4
引  言    5
1 材料和方法    6
    1.1 實驗儀器和試劑     7
    1.2供試材料及其培養    8
1.2.1藻種培養    8
1.2.2重金屬處理    8 鈷對集胞藻6803生長的影響研究:http://www.aftnzs.live/shengwu/20190414/32048.html
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