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紫蘇醛對白色念珠菌的細胞周期和細胞凋亡的影響

時間:2019-04-13 14:33來源:畢業論文
研究紫蘇醛藥液抗白色念珠菌的機制,觀察不同濃度的紫蘇醛藥液對白色念珠菌的甾醇含量、細胞膜損傷,以簡單研討紫蘇醛對真菌細胞膜的作用機理。用紫外可見分光光度測量麥角固

摘要:控制白色念珠菌引起的真菌感染是臨床上較為棘手的一類難題,研究并開發新的有效的抗白色念珠菌藥物成為了近年來抗真菌藥物研發重點之一,而紫蘇醛是一種成效顯著、廣譜、低毒的抗菌藥物。本實驗主要研究紫蘇醛藥液抗白色念珠菌的機制,觀察不同濃度的紫蘇醛藥液對白色念珠菌的甾醇含量、細胞膜損傷,以簡單研討紫蘇醛對真菌細胞膜的作用機理。用紫外可見分光光度測量麥角固醇含量,用流式細胞儀檢測細胞膜損傷,結果顯示紫蘇醛可作用于細胞膜,抑制細胞膜中麥角固醇的合成,膜的通透性改變,損傷細胞膜。用流式細胞儀檢測分析細胞周期和細胞凋亡,結果表明,紫蘇醛能使細胞周期阻滯在G2/M期,誘導細胞發生凋亡。綜上所述,不同濃度的紫蘇醛均(0-0.4 µL/mL)對白色念珠菌的生長具有的抑制作用,抑制效果和濃度成正比,抗真菌機制主要歸納為抑制細胞膜中麥角固醇的合成導致細胞膜受損、阻滯細胞周期和誘導細胞凋亡。34461
畢業論文關鍵詞:紫蘇醛;白色念珠菌;細胞周期;細胞凋亡
Influence ofperillaldehydeon cell cycle and apoptosis of Candida albicans
Abstract:Control the fungal infection caused byC.albicans is relatively difficult clinical problem. Consequently,research and development of new effective method of resistance of C.albicans has become one of the emphases on the antifungal drugs in recent yearsan,andperillaldehyde is a kind of effective, broad-spectrum, low toxicity of antibacterial drugs. Thisexperiment mainly study perillaldehyde solution mechanism of resistance of C.albicans,at different concentrations of perillaldehyde solution of C.albicans sterol content, cell membrane injury, in a simple discussion perillaldehyde on the mechanism of the fungal cell membrane. Ergosterol content is measured with a UV-spectrophotometer,usingflowcytometryinstrumentdetectioncell membrane damage, the results show that the perillaldehyde can be applied to the cell membrane, the synthesis of ergosterol in inhibition of cell membrane, change the permeability of membrane, damage the cell membrane. Using flow cytometry instrument testing analysis of cell cycle and cell apoptosis, test results indicate that, perillaldehyde can make the cell cycle arrest in GO/G1 phase, induction of cell apoptosis. In conclusion, different concentrations of perillaldehyde (0-0.4µL/mL) on the growth of C.albicans has inhibitory effect, inhibition effect and is directly proportional to the concentration, antifungal mechanism is mainly attributed to inhibit the synthesis of ergosterol in the cell membrane, destroy the integrity of the cell membrane, block the cell cycle and inducing apoptosis. 源¥自%六^^維*論-文+網=www.aftnzs.live
Key words:Perillaldehyde; C.albicans; Cell cycle; Apoptosis
目錄
引言    1
1 材料與方法    3
1.1供試材料    3
1.1.1原料    3
1.1.2實驗試劑與儀器    3
1.1.3 培養基    4
1.1.4 各種試劑溶液的配制    5
1.2 實驗方法    6
1.2.1 白色念珠菌菌種活化    6
1.2.2 菌懸液的制備    6
1.2.3 微孔稀釋法測定最低抑菌濃度(Minimum Inhibitory Concentration, MIC)    6
1.2.4紫蘇醛對白色念珠菌甾醇含量的影響(Sterol Quantitation Method, SQM)    6
1.2.5紫蘇醛對白色念珠菌細胞膜損傷的影響    7
1.2.6 紫蘇醛對白色念珠菌細胞周期的影響    7
1.2.7 紫蘇醛對白色念珠菌細胞凋亡的影響    8
2 結果與分析    9
2.1紫蘇醛對白色念珠菌的MIC值    9
2.2紫蘇醛對白色念珠菌甾醇含量分析    9
2.3紫蘇醛對白色念珠菌細胞膜損傷測量    10 紫蘇醛對白色念珠菌的細胞周期和細胞凋亡的影響:http://www.aftnzs.live/shengwu/20190413/31967.html
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