畢業論文

打賞
當前位置: 畢業論文 > 生物論文 >

鴨PRKAB1基因多態性研究

時間:2019-04-13 14:31來源:畢業論文
檢測了PRKAB1基因的8個基因座,其中僅PRKAB1-1基因座表現有SNP位點,而其余的七個基因座均呈單態。根據位點多態性可分成AA、AB、BB三種基因型,其中A等位基因的頻率為0.6148,B為0.3852

摘要:本試驗以北京鴨為材料,根據鴨PRKAB1基因序列設計引物,采用了PCR-SSCP技術和DNA測序技術,檢測了北京鴨共122個個體PRKAB1基因單核苷酸多態性,分析了北京鴨的該基因的遺傳結構多樣性與其飼料轉化率的相關性,從而檢驗這個基因的多態對北京鴨飼料轉化率的遺傳效應,希望能夠發現對飼料轉化率有顯著效應的遺傳標記,以便提高肉鴨的選育的效率,并可以為分子標記數據庫的建立和種質資源保護等提供遺傳學依據[1]。本研究共檢測了PRKAB1基因的8個基因座,其中僅PRKAB1-1基因座表現有SNP位點,而其余的七個基因座均呈單態。根據位點多態性可分成AA、AB、BB三種基因型,其中A等位基因的頻率為0.6148,B為0.3852。經過卡方合適性檢驗,發現各基因型之間不符合Hardy - Weinberg平衡定律。通過本試驗可以發現在北京鴨群體中,PRKAB1基因座不同基因型個體與飼料轉化率存在相關性(P<0.05),且AB基因型個體料重比水平顯著高于BB基因型個體(P<0.05)。34459
畢業論文關鍵詞:北京鴨、SNP、PRKAB1基因、多態性、飼料轉化率
The Polymorphism of PRKAB1 Gene in Duck
Abstract: In this study Peking duck were used as the test material and primers were designed according to the sequence of PRKAB1 gene.We used the PCR-SSCP technique and DNA sequencing technology to detect the single nucleotide polymorphisms of Peking duck’s PRKAB1 gene in 122 inpidual . The Peking Duck genetic structure of the gene and its correlation persity was analyzed, so that to examine the association between polymorphism of this gene and effects of genotypes of candidate genes on feed conversion rate of Peking duck, hoping to find the feed conversion rate has a significant effect of genetic markers, in order to improve feed conversion rate of breeding ducks, and to provide genetic information for foundation of molecular marker database, protection and usage of breed resource of Peking duck[1]. This study examined eight loci PRKAB1 gene locus which only PRKAB1-1 manifestations of the SNP, while the remaining seven loci showed a single state. According polymorphism can be pided into AA, AB, BB three genotypes, and allele frequency of A is 0.6148, B is 0.3852. After  chi-square proper test ,we found the genotypes does not meet Hardy - Weinberg equilibrium. Through this experiment ,we can found that in Peking duck , the duck of different genotypes in PRKAB1 loci were related to feed conversion rate (P <0.05) and , and AB genotypes feed weight ratio was significantly higher than BB genotype (P < 0.05). 源¥自%六^^維*論-文+網=www.aftnzs.live
Keywords: Peking duck, SNP, PRKAB1 gene, polymorphism, feed conversion rate
目錄
前言    1
1材料與方法    2
1.1 試驗材料    2
1.1.1 試驗動物    2
1.1.2 試驗主要試劑    2
1.1.3 溶液與緩沖液    2
1.1.4 儀器設備    3
1.2 試驗方法    3
1.2.1 血樣的采集    3
1.2.2體尺指標的獲得    3
1.2.3鴨全血基因組DNA的提取[14]    3
1.2.4 DNA質量的檢測、純化及濃度計算    3
1.2.5 PCR反應所用引物的設計    3
1.2.6 PCR-SSCP DNA標記分析    4
1.3統計分析方法    5
1.3.1 基因頻率和基因型頻率的計算    5
1.3.2 基因頻率和基因型頻率的差異顯著性檢驗    5
1.3.3 不同基因型的個體之間產肉性狀的相關分析及顯著性檢驗    6
2結果與分析    6
2.1 北京鴨基因組DNA樣品的檢測    6
2.2 北京鴨PRKAB1基因座結果與分析    6
2.2.1 PRKAB1基因座 PCR擴增結果及SSCP 分析    6 鴨PRKAB1基因多態性研究:http://www.aftnzs.live/shengwu/20190413/31965.html
------分隔線----------------------------
推薦內容
双色球走势图带连线