畢業論文

打賞
當前位置: 畢業論文 > 生物論文 >

鴨PRKAA2基因多態性研究

時間:2019-04-07 20:30來源:畢業論文
采用PCR-SSCP和DNA測序方法首次分析了PRKAA2基因在我國北京鴨中的遺傳多態性,從而分析北京鴨它的遺傳結構及其多態性與飼料利用率的相關性

摘要:PRKAA2基因是單核苷酸活化蛋白激酶(AMPK)的α2亞基基因,本研究采用PCR-SSCP和DNA測序方法首次分析了PRKAA2基因在我國北京鴨中的遺傳多態性,從而分析北京鴨它的遺傳結構及其多態性與飼料利用率的相關性,近而發現那些具有重要經濟性狀的個體所具有的獨特的遺傳特點,為北京鴨的選育提供顯著的遺傳標記,同時為基因標記數據庫的建立提供遺傳學依據。實驗中采用PCR-SSCP方法對北京鴨PRKAA2基因進行分析,結果表明PRKAA2基因存在多種多態性形式,后來結合DNA測序加以分析,共發現了2個多態性位點中存在的5個突變位點。第2位點存在兩種基因型,分別是AA和AB型,其頻率分別為0.29和0.71。 A等位基因頻率為0.645,B等位基因頻率為0.355。第6位點存在三種基因型,分別是AA、AB和BC型,其頻率人別為0.305、0.555和0.14,A等位基因頻率為0.5825,B等位基因頻率為0.3475,C等位基因頻率為0.07,且χ2 適合性檢驗結果表明,PRKAA2基因的第2和第六2個位點的各基因型均不符合Hardy - Weinberg平衡定律。 34361
畢業論文關鍵字:北京鴨;PRKAA2基因;PCR-SSCP;分子遺傳特征分析;
注:4位點和8位點的等位基因A、B是不同的。
Research on the polymorphisms of PRKAA2 Gene in Duck
Abstract: PRKAA2 gene is alpha 2 subunit gene of single nucleotide amp-activated protein kinase, the PCR-SSCP and DNA sequencing method are adopted to analyze the genetic polymorphisms of PRKAA2 gene in Peking duck for the first time, so as to analyze Peking duck its genetic structure and the correlation between polymorphism and feed utilization rate. the researchs are used to find important economic characters of the inpidual that it has unique genetic characteristics. these founds are beneficial for efficient breeding of Peking duck in China and the construction of gene marker database. Peking duck PRKAA2 gene was analyzed by using PCR-SSCP method, and the results show that the PRKAA2 genes exist multiple genetic polymorphism. Then the results are proved more by DNA sequencing.finally, 2 polymorphism locis and five SNP are found. The 2nd loci has two genotypes they are AA and AB, and the frequency of them are 0.29 and 0.71 respectively.A allele frequency is 0.645, B allele frequency is 0.355. The 6ix loci has three genotypes they are AA, AB and BC, the frequency of them are 0.305, 0.555 and 0.14. A allele frequency is 0.5825, B allele frequency is 0.3475, C allele frequency is 0.07. χ2 test results showed that genotypes of 2 locis in PRKAA2 gene do not consistent with Hardy- Weinberg equilibrium. 源¥自%六^^維*論-文+網=www.aftnzs.live
Key words: Peking Duck; PRKAA2; PCR-SSCP; Molecular Genetic Analysis
  Note:the A and B of 4 site and 8 site is different.
目  錄
引言    1
1.我國北京鴨生產與育種發展現狀    1
2. PRKAA2基因    2
1實驗材料與實驗方法    2
1.1實驗材料    2
1.2實驗藥品和試劑    2
1.2.1普通試劑    2
1.2.2分子生物學試劑    3
1.2.3 主要溶液與緩沖液配制    3
1.3 試驗方法    3
1.3.1血樣的采集    3
1.3.2 鴨全血基因組DNA的提取    3
1.3.3 PCR反應所用引物的設計    3
1.3.4 DNA質量的檢測、純化及濃度計算    4
1.3.5 PCR最佳反應條件的篩選    4
1.3.6瓊脂糖凝膠電泳檢測DNA    5
1.3.7擴增產物的PCR-SSCP分析    5
1.3.8 DNA序列測定    5
1.3.9 統計分析方法    5
2結果與分析    6
2.1從血液中提取基因組DNA    6
2.2 PCR擴增結果和PCR最佳反應條件的篩選….6 鴨PRKAA2基因多態性研究:http://www.aftnzs.live/shengwu/20190407/31834.html
------分隔線----------------------------
推薦內容
双色球走势图带连线