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徐州魚塘細菌多樣性研究

時間:2019-04-07 20:14來源:畢業論文
通過對鯽魚魚塘水體中細菌的總DNA進行提取,經過PCR擴增并依據16S rRNA基因序列構建系統發育樹,進行分子發育學研究,最后獲得魚塘水體中細菌物種的組成

摘要:魚塘的健康養殖受到多種因素的制約,如魚的種類以及魚塘的面積大小等,同時,魚塘水體中的細菌對魚類存活更是帶來重大影響。本文對徐州北郊鯽魚魚塘水體中細菌的多樣性及致病菌對該魚塘中鯽魚存活狀況的影響進行了研究,進一步實現魚塘的科學管理。研究主要是通過對鯽魚魚塘水體中細菌的總DNA進行提取,經過PCR擴增并依據16S rRNA基因序列構建系統發育樹,進行分子發育學研究,最后獲得魚塘水體中細菌物種的組成。它們分別為崛越氏芽孢桿菌(Bacillus horikoshii)、Aeromonas punctata、Bacillus thaonhiensis、溶血葡萄球菌(Staphylococcus haemolyticus)、弗氏志賀菌(Shigella flexneri)、印度芽孢桿菌(Bacillus indicus)、Fictibacillus phosphorivorans、Enterobbacter soli、Bacillus aryabhattai。經檢測其中Aeromonas punctata、弗氏志賀菌(Shigella flexneri)及溶血性葡萄球菌(Staphylococcus haemolyticus)為致病菌。研究徐州北郊魚塘水體中細菌多樣性及其中致病菌對魚類存活狀況的影響不僅可以補充完善我國的魚塘養殖環境中微生物物種資源,還為魚塘健康養殖提供重要的參考依據。34355
畢業論文關鍵詞:多樣性;陽性菌;陰性菌;致病菌;16S rRNA;系統發育樹   
Study on bacterial persity in fish pond water in the north of Xuzhou
Abstract:Healthy breeding of fish ponds restricted by many factors, such as the types of fish and fish ponds area size, etc. At the same time, the bacteria in fish pond water for fish survival but also bring significant impact .This paper studies the influences of different bacteria to different fishes in the fish pond in the north of Xuzhou. The research is mainly through 16S rRNA gene sequencing of the fish pond water bacteria in the north of Xuzhou, and the 16S rRNA gene sequence analysis of phylogenetic tree construction, finally get the composition of bacterial species in water. After testing , We found that there were nine kinds of bacteria in fish pond water, they were Bacillus horikoshii、Aeromonas punctata、Bacillus thaonhiensis、Staphylococcus haemolyticus、Shigella flexneri、Bacillus indicus、Fictibacillus phosphorivorans、Enterobbacter soli、Bacillus aryabhattai. The Aeromonas punctata、Staphylococcus haemolyticus and Shigella flexneri were pathogenic bacteria.Study on the effect of bacterial persity and pathogen on fish survival status of the fish pond water in the north of Xuzhou can not only improve our microbial species resources of aquiculture environment, but also provide an important reference for the healthy breeding of the fish ponds. 源¥自%六^^維*論-文+網=www.aftnzs.live
Key words: persity; Gram-negative bacteria; Gram-positive bacteria; pathogenic bacteria; 16S rRNA; Phylogenetic tree
目錄
引言    1
1材料與方法    2
1.1    材料    2
1.1.1    水樣采集    2
1.1.2    配制培養基    2
1.1.3    主要儀器與試劑    2
1.2  方法    2
1.2.1    對菌種進行劃線分離與純化并斜面保存    2
1.2.2    Gram stain染色    2
1.2.3    細菌總DNA抽提及電泳分析    2
1.2.4    細菌的PCR擴增    2
1.2.5    PCR擴增產物電泳分析    3
1.2.6    擴增產物膠回收    3
1.2.7    測序    3
1.2.8    構建系統發育樹    3
2結果與分析    3
2.1    革蘭氏染色結果    3
2.2    系統發育樹    3
3討論    5 徐州魚塘細菌多樣性研究:http://www.aftnzs.live/shengwu/20190407/31828.html
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