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抗植物病原真菌放線菌的篩選

時間:2019-04-07 09:22來源:畢業論文
以稻曲病病原菌(Ustilaginoidea virens(Cooke) Takah.)、黃瓜根腐病病原菌(Fusarium solani (Mart.) Sacc.)、蘋果輪紋病病原菌(Botryosphaeria berengeriana de Not. f. sp. Parabola Nise Koganezawa et Sakuma)

摘要:植物病原真菌嚴重影響農業生產,本文以稻曲病病原菌(Ustilaginoidea virens(Cooke) Takah.)、黃瓜根腐病病原菌(Fusarium solani (Mart.) Sacc.)、蘋果輪紋病病原菌(Botryosphaeria berengeriana de Not. f. sp. Parabola Nise Koganezawa et Sakuma)、水稻紋枯病病原菌(Rhizoctonia solani Kühn)、西瓜枯萎病病原菌(Fusarium oxysporum Schl. f. sp. niveum (E.F.Smith) Snyder et Hansen)、水稻惡苗病病原菌(Fusarium moniliforme Sheld.)為篩選模型,對來自四川攀枝花麻瘋樹和新疆土壤中的部分放線菌菌株進行篩選,以期得到具有生防價值的菌株。本文通過劃線法對實驗室保存的菌株進行活化,共活化出13株放線菌;利用平板對峙法進行初篩,共篩選出7株具有活性的放線菌,編號分別為Z01、Z04、Z06、Z09、Z15、Z18和Z23,其中編號為Z01、Z04、和Z18這三種放線菌抑菌效果明顯,活性較強;采用牛津杯法對初篩得到的有活性的菌株進行發酵液復篩,結果表明:Z04和Z18的發酵液對部分供試真菌具有抑制作用;最后,利用氣質譜聯用技術對Z04的發酵液乙酸乙酯提取物的化學成分進行分析,共得到32個色譜峰,分屬于芳香烴類,酚類,酯類,脂肪酸類及含氮化合物。本研究為下一步生物農藥的開發提供了一定的實驗數據。34328
畢業論文關鍵詞:植物病原菌;放線菌;活性—化學集成篩選;次級代謝產物;生物防治
Screening of actinomycetes against plant pathogenic fungi
Abstract: Plant pathogenic fungi seriously affected the agricultural production. In order to get high value of biocontrol strains, Some actinomycetes from Jatropha of Sichuan Panzhihua and soil of Xinjiang were screened again Ustilaginoidea virens(Cooke) Takah, Fusarium solani (Mart.) Sacc, Botryosphaeria berengeriana de Not. f. sp. Parabola Nise Koganezawa et Sakuma, Rhizoctonia solani Kühn, Fusarium oxysporum Schl. f. sp. niveum (E.F.Smith) Snyder et Hansen and Fusarium moniliforme Sheld. In this paper, 13 kinds of actinomycetes were activated by crossing line method. 7 kinds of actinomycetes with antifungal activity were screened out using plate confrontation method The 7 strains were Z01, Z04, Z06, Z09, Z15, Z18, Z23, respectively. The activity of Z01, Z04, and Z18 were significantly stronger. Their fermentation broth were double-checked by the Oxford cup method. The result showed that the fermentation liquid of Z18 and Z04 had inhibitory effect on the partial test fungi. Finally, the chemical constituents of the fermentation broth of Z04 were extractd using ethyl acetate. The ethyl acetate extracts were analyzed by spectrum technology. There were 32 peaks belonging to aromatic hydrocarbons, phenols, esters, fatty acids and nitrogen-containing compounds. This study provided some experimental data for the next step in the development of biological pesticides. 源¥自%六^^維*論-文+網=www.aftnzs.live
Key words: Plant pathogens; Actinomycetes; Activity-chemical integrated screening; Secondary metabolites; Biological control
目錄
摘   要    Ⅰ
Abstract    Ⅰ
引言    1
1材料與方法    2
1.1材料    2
1.1.1放線菌菌株    2
1.1.2植物病原真菌    2
1.1.3實驗儀器    3
1.2方法    3
1.2.1常用培養基的配制    3
1.2.2放線菌的純化、保種    4
1.2.3抗植物病原真菌的活性菌株初篩    4
1.2.4抗植物病原真菌的發酵液復篩    4
1.2.5發酵液的化學篩選    5
2結果與分析    5
2.1放線菌的活化結果    5
2.2 平板對峙法初篩結果與分析    6
2.3發酵液抑菌復篩結果與分析    9 抗植物病原真菌放線菌的篩選:http://www.aftnzs.live/shengwu/20190407/31798.html
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