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珍珠粉對果蠅抗熱激及生殖能力的影響

時間:2019-04-04 20:39來源:畢業論文
探討不同濃度的珍珠粉在熱激脅迫下對果蠅壽命及在常溫下對其生殖能力的影響.方法:收集同一批8h內羽化但未交配的野生型黑腹果蠅, 運用乙醚麻醉的方法,將果蠅等量分裝于含不同濃度

摘要:目的:探討不同濃度的珍珠粉在熱激脅迫下對果蠅壽命及在常溫下對其生殖能力的影響.方法:收集同一批8h內羽化但未交配的野生型黑腹果蠅, 運用乙醚麻醉的方法,將果蠅等量分裝于含不同濃度的珍珠粉的固體培養基中。30℃下培養觀察并統計果蠅的存活數量;同步化處理果蠅選擇8h內羽化但未交配的果蠅,雌雄各半。25℃下培養,觀察并記錄其蛹化的數量及速度。結果:熱脅迫下低劑量組(每100g基礎培養基中含有0.01g珍珠粉,即0.01%)和中劑量組(0.1%)的果蠅壽命比對照組(不含珍珠粉)高且低劑量更明顯,高劑量組(1%)果蠅的壽命低于對照組。常溫下低劑量產卵數量與速度高于對照組,而高劑量則低于對照組。結論:適當劑量珍珠粉可以促進果蠅的生長發育及抗熱激能力,高濃度時則對抑制果蠅的生長發育及抗熱激能力。34207
畢業論文關鍵詞:珍珠粉;果蠅;壽命;生殖能力; 生長發育
The impact of pearl powder on resistance to heat shock and the reproductive capacity of Drosophila
Abstact: Objective:To explore the impact of different concentrations of pearl power on the lifespan of Drosophila under heat shock stress and the reproductive capacity of Drosophila at room temperature. methods :Gathering with a group of 8h emergence but not mating wild-type Drosophila melanogaster, choosing the appearance of normal and inpidual size similar to the Drosophila as the research object by using ether anesthesia method, Same amount of fruit flies are randomly put into the solid medium with different concentrations of pearl power. Grown in 30 degree, the statistics of Drosophila melanogaster and the number of surviving were observed daily in the same time; We should choice a group of 8h emergence but not mating wild-type Drosophila melanogaster from synchronized Drosophila, male and female half . The flies are cultivated under 25 degree. Then we should observe the situation of the offspring propagation. Life span of fruit in the low dose group(per 100 grams of basic culture medium containing pearl powder 0.01 grams,0.01%) and medium dose group(0.1%) under heat stress are higher than that of the control group(no pearl powder), and that of the low dose is highest, but that of the high dose group(1%)  is lower than that of the control group .The number and speed of spawn in the low dose group and medium dose group under normal temperature are higher than that of the control group, and that of the low dose is highest, but that of the high dose group is lower than that of the control group. Conclusion: Appropriate doses of pearl powder have a certain role in promoting in the growth and development of Drosophila under heat stress, but with the increasing of the concentration, the growth of Drosophila development has a slow trend. 源¥自%六^^維*論-文+網=www.aftnzs.live
Keywords: pearl powder; Drosophila; life; reproductive capacity; growth and development 
目    錄
摘要    1
引言    2
1 材料與方法    3
1.1實驗材料    3
1.2主要實驗儀器    3
1.3 實驗方法    3
1.3.1基礎培養基的配置    3
1.3.2 實驗培養基的配置    3
1.3.3果蠅的麻醉    4
1.4實驗指標的測定    4
1.4.1 高溫下珍珠粉對果蠅壽命的影響的測定    4
1.4.2常溫下珍珠粉對果蠅生殖能力的影響    4
1.4.3數據處理    5
2結果    5
2.1熱脅迫下珍珠粉對果蠅壽命的影響    5
2.1.1 熱脅迫下各組果蠅的壽命情況    5
2.1.2 熱脅迫下果蠅的行為    7
2.2珍珠粉對果蠅生殖能力的影響    8
2.2.1  25℃下珍珠粉對果蠅生殖能力的影響的實驗數據    8 珍珠粉對果蠅抗熱激及生殖能力的影響:http://www.aftnzs.live/shengwu/20190404/31661.html
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