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茶樹精油微膠囊的制備和性能研究

時間:2019-05-05 21:19來源:畢業論文
為制備得到具有良好的穩定性的茶樹油精油微膠囊,且研究茶樹精油微膠囊的抑菌能力。本實驗采用乳化包埋法制作納米顆粒,選用變量控制為壁材配比,攪拌時間30-60min作為考察時間

摘要:本課題為制備得到具有良好的穩定性的茶樹油精油微膠囊,且研究茶樹精油微膠囊的抑菌能力。本實驗采用乳化包埋法制作納米顆粒,選用變量控制為壁材配比,攪拌時間30-60min作為考察時間,pH1.5-9.5作為考察pH,選取茶樹精油為芯材,分別試驗了甜茶提取物,明膠,阿拉伯膠,β-環糊精和麥芽糊精,單獨或配比使用時對于茶樹精油的包埋作用。采用濾紙片擴散法,以大腸桿菌、產黃青霉為指示菌,測定茶樹精油和茶樹精油微膠囊的抑菌活性。用不同濃度的茶樹精油和茶樹精油微膠囊做最小抑菌濃度(MIC)測定。結果表明:采用甜茶提取物和麥芽糊精為壁材,控制壁材配比為5:5,芯材壁材配比為10:1,攪拌時間為60min,體系pH為5.5,制得了粒徑80nm,包埋率達到了85%的茶樹油精油微膠囊,該茶樹油精油微膠囊具有良好的穩定性。同時茶樹精油微膠囊的抑菌能力與茶樹精油相當,對產黃青霉的抑菌作用較強,對大腸桿菌的抑菌作用較弱。35195
畢業論文關鍵詞:茶樹油精油;乳化包埋;微膠囊;壁材;抑菌;
Preparation and Properties of Tea Tree Oil Microcapsules
Abstract:For the preparation of this paper to give a good stability of tea tree oil microcapsules and study antibacterial activity of tea tree oil microcapsules. In this study, the production of emulsion embedding nanoparticles, use variable control as wall material ratio, mixing time 30-60min as study time, pH1.5-9.5 as study pH, choose tea tree oil as core material were tested Rubusoside material, gelatin, gum arabic, β- cyclodextrin and maltodextrin, alone or when used for the ratio of embedding the role of tea tree oil. Filter paper diffusion method, E. coli, P. chrysogenum as indicative bacteria, antibacterial activity was measured tea tree oil and tea tree oil microcapsules. With different concentrations of tea tree oil and tea tree oil microcapsules do the minimum inhibitory concentration (MIC) determination. The results show that: sweet tea extract and maltodextrin as wall material, wall material ratio control is 5: 5, core wall material ratio of 10: 1, stirring time is 60min, the pH of 5.5, was prepared grain 80nm , embedding rate reached 85% of the tea tree oil microcapsules, the tea tree oil microcapsules have good stability. Meanwhile antibacterial activity of tea tree oil and tea tree oil microcapsules equivalent chrysogenum inhibitory effect on strong inhibitory effect on E. coli weak. 源¥自%六:維;論-文'網=www.aftnzs.live
Key Words: Tea tree oil; emulsified embedding; microcapsule; wall material; antibacterial;
 目錄
1 緒論    6
1.1 茶樹精油概述    6
1.2 微膠囊制作方法    6
1.2.1 乳化包埋法    6
1.2.2 高壓均質法    7
1.2.3 薄膜法制備脂質體    7
1.2.4 噴霧干燥法    8
1.3 主要壁材介紹    8
1.3.1 甜茶提取物    8
1.3.2 麥芽糊精    8
1.3.3 明膠    9
1.3.4 阿拉伯膠    10
1.3.5 β-環糊精    10
1.4 本課題的研究目的和意義    10
2 實驗部分    11
2.1 實驗材料    11
2.1.1 實驗材料和試劑    11
2.1.2 主要儀器和設備    12
2.2 茶樹精油成分分析    12
2.2.1 氣相色譜法    12
2.2.2 高效液相法    12
2.3 茶樹精油微膠囊的制備工藝研究    13
2.3.1 壁材的篩選    13
2.3.2 茶樹精油微膠囊的單因素實驗    14
2.4 抑菌實驗    15
2.4.1 實驗流程    15
2.4.2 操作要點    15 茶樹精油微膠囊的制備和性能研究:http://www.aftnzs.live/huaxue/20190505/33040.html
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