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迷迭香有效成分的提取及其抗菌功效評價

時間:2019-04-21 09:06來源:畢業論文
以迷迭香作為實驗原料,采用超聲波提取技術,對迷迭香有效成分進行提取,通過單因素實驗分別考察了提取溶劑、提取時間以及料液比對迷迭香有效成分提取率的影響

摘要:迷迭香在藥品、食品等多個方面都有很好的應用前景。本文以迷迭香作為實驗原料,采用超聲波提取技術,對迷迭香有效成分進行提取,通過單因素實驗分別考察了提取溶劑、提取時間以及料液比對迷迭香有效成分提取率的影響。并采用抑菌圈和最小抑菌濃度法考察迷迭香有效成分的抑菌效果。結果表明:迷迭香有效成分最佳提取條件是,常溫條件下,用75%乙醇,物料比為1:28時超聲提取25min。此時迷迭香提取物的提取率最高。所提取的迷迭香提取物其抑菌效果隨著迷迭香提取物濃度的升高而增強。按照試驗成果表明,6%濃度的迷迭香提取物迷迭香酸抑菌效果最好。34777
畢業論文關鍵詞:迷迭香;迷迭香酸;抑菌;提取率
Extraction of active constituents from rosemary and its antibacterial effect evaluation
Abstract: Rosemary's value has been proven in many aspects of drugs, food, etc.. There are many good prospects of application results in these areas. In this paper, I will use five concentrations ethyl alcohol in 15%, 35%, 55%, 75%, 95% as a solvent extraction process, the active ingredient of rosemary research, based on the extraction of the active ingredient in rosemary fans Rosemary acid, and inhibition zone method to evaluate its antimicrobial efficacy with the lowest concentration of extract of E. coli inhibition study its application in matrix cream and other cosmetics. The results show that: The best extraction condition of rosemary active ingredient was: under normal temperature condition, 1:28 when the ultrasonic extraction was 25min and 75% ethanol. The extraction rate of rosemary extract was the highest at that situation. The extracts of the rosemary extract were enhanced with the increase of the concentration of the extract. According to the results of the experiment, the antibacterial effect of the 6% concentration of rosemary extract is the best.

源¥自%六:維;論-文'網=www.aftnzs.live


Rosemary have proved some application in cosmetics antibacterial, it also can be used as plant extracts added to cosmetics.
Key word:  rosemary; rosemary acid; bacteriostat;extraction rate
目錄
1 前言    1
1.1 迷迭香國內外研究現狀    1
1.1.1 迷迭香有效成分    1
1.1.2 迷迭香酸的性質    1
1.2 迷迭香酸的研究方法    2
1.2.1 超聲波輔助提取法提取迷迭香酸    2
1.2.2 超臨界二氧化碳萃取法提取迷迭香酸    2
1.2.3 纖維素酶輔助提取法提取迷迭香酸    2
1.2.4 溶劑法提取迷迭香酸    3
1.2.5 常規熱回流提取法提取迷迭香酸法    3
1.2.6 微波法提取迷迭香酸    3
1.2.7 pH控制勻漿法提取迷迭香酸    3
1.3 抑菌研究方法現狀    4
1.3.1 紙片擴散法    4
1.3.2 稀釋法    4
1.3.3 杯碟法杯    4
1.3.4 瓊脂稀釋法    4
1.4 本課題研究的目的和意義    5
2 實驗原理與方法    5
2.1 實驗原料與儀器設備    5
2.1.1 實驗原料    5
2.1.2 實驗儀器與設備    7
2.2 實驗步驟    9
2.2.1 迷迭香粉末制備    9
2.2.2 迷迭香酸提取效率的探究    9
2.2.3 含迷迭香提取物乳液的配制    10
2.2.4 含迷迭香成分乳液性能的測試    12
2.3 迷迭香提取物抑菌效果的測定    12 源¥自%六:維;論-文'網=www.aftnzs.live
2.3.1 配制培養基    12
2.3.2 菌種的準備    13
2.3.3 抑菌圈滅菌法    13
2.3.4 最小抑菌濃度法    14 迷迭香有效成分的提取及其抗菌功效評價:http://www.aftnzs.live/huaxue/20190421/32422.html
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