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介孔SiO2載藥微球的合成與性能初探

時間:2019-06-09 14:35來源:畢業論文
探究兩種載藥系統對癌細胞的滅殺情況。結果表明,較之MSNs,PEG修飾后的MSNs微球具有更小粒徑(~100nm)、更大孔容和更高的比表面積等特點,并具有較高的載藥率和包封率

摘要 介孔二氧化硅(MSNs)作為一類新型的藥物載體在藥物緩控釋領域的應用方興未艾。本課題采用模板誘導和自組裝方法合成MCM-41型介孔二氧化硅納米顆粒,并通過聚乙二醇(PEG)的表面修飾,制備出一類具有高親水性、高孔隙率、高比表面積的MSNs修飾微球。采用氮吸附(BET)、掃描電鏡(SEM)、透射電鏡(TEM)、粒徑分析等手段表征并對比了聚乙二醇(PEG)修飾前后的MSNs基礎理化性能。在此基礎上,選用阿霉素作為模型藥物,包埋于介孔材料中,對比了PEG修飾前后MSNs中阿霉素的裝載量、包封率及釋放行為。選用乳腺癌細胞MCF-7為模型癌細胞,人血清蛋白(HAS)為模型蛋白,考察了兩種材料對細胞的生物安全性及對蛋白的吸附情況,并將兩種材料所對應的載藥微球與MCF-7進行共培養,探究兩種載藥系統對癌細胞的滅殺情況。結果表明,較之MSNs,PEG修飾后的MSNs微球具有更小粒徑(~100nm)、更大孔容和更高的比表面積等特點,并具有較高的載藥率和包封率。由于PEG的修飾,載藥MSNs微球具有更加明顯的緩釋特性,且其藥物作用時間更長,滅殺癌細胞效果更好。36099
畢業論文關鍵詞:  介孔二氧化硅;PEG表面修飾;藥物緩控釋系統;生物安全性;抗癌
Synthesis and characterization of mesoporous silica nanoparticles for drug delivery Abstract As a new type of drug carriers,Mesoporous silica  nanoparticles  (MSNs) is in the ascendant in the field of drug control and release. In this article, both the template induced and self-assembling method was employed to synthesize the MCM-41 type of mesoporous silica nanoparticles. With the co-polymerization with polyethylene glycol (PEG),  MSNs  with wormhole pore structure were obtained with high hydrophilic, high porosity and specific surface area. The modification of PEG to the MSNs was characterized by nitrogen adsorption (BET), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and particle size analysis. Besides, the influence of PEG modification to the drug loading content, the encapsulation efficiency and the release behavior of the MSNs were also analyzed with the doxorubicin as a model drug. To compare the biosecurity and protein adsorption of the two kinds of materials, the human breast cancer cell MCF-7 and the human serum albumin (HAS) were selected as the model cancer cells and the model protein, respectively. On the other hand, to investigate the anti-cancer effect of these two drug delivery systems, the materials were co-cultured with MCF-7 cells and determined the quantity of cells by means of Microplate Reader. The results show that, compared with unmodified MSNs, MSNs-PEG were spherical morphology with smaller size (100 nm), larger pore volume and higher specific surface area. And with the PEG modification,  MSNs  has higher drug loading rate and entrapment rate. Owing to the modification of PEG, the drug loaded MSNs microspheres have more evident of controlled-release properties and better anticancer properties. 源¥自%六:維;論-文'網=www.aftnzs.live
KeyWords:  Mesoporous Silica Nanoparticles; PEG modification; Sustained and Controlled Release Drug Delivery; Biosecurity; Anticancer
目錄
1文獻綜述..1
1.1引言.1
1.2納米靶向載藥系統.1
1.2.1納米靶向載藥系統的定義1
1.2.2納米靶向載藥系統的分類2
1.2.3納米靶向載藥系統的優勢3
1.3緩控釋載藥系統.3
1.3.1藥物的緩釋與控釋3
1.3.2緩控釋載藥系統制劑的研究進展3
1.4介孔二氧化硅納米顆粒.4
1.4.1MSNs材料的合成機理.5
1.4.2MSN制備工藝的研究進展..6
1.5基于MSNs表面功能化的藥物控釋7
1.5.1酸性基團修飾MSNs的藥物控釋.8
1.5.2堿性基團修飾MSNs的藥物控釋.8
1.5.3中性基團修飾MSNs的藥物控釋.8
1.6本課題研究的目的和意義.9 介孔SiO2載藥微球的合成與性能初探:http://www.aftnzs.live/cailiao/20190609/34428.html
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